| 切花菊组织培养快速繁殖技术 |
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| 引用本文： | 程广有,韦立文.切花菊组织培养快速繁殖技术[J].延边大学农学学报,2000,22(3):203-206. |
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| 作者姓名： | 程广有 韦立文 |
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| 作者单位： | [1]北华大学林学院,吉林 [2]吉林市林业职业中等专业学校,吉林 |
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| 摘 要： | 在ＭＳ培养基基础上，添加不同种类和浓度的激素，对切花菊进行组织培养快速繁殖技术的研究，外植体分别为腋芽，茎段，花蕾，叶片，叶炳。其中以带芽茎段，叶柄和腋芽的增殖效果较好，诱导不定芽的培养基以ＭＳ＋６－ＢＡ１．０ｍｇ／Ｌ＋ＮＡＡ０．１ｍｇ／Ｌ不定芽的发生率最高。不定根的诱导以１／２ＭＳ＋ＩＢＡ１．０ｍｇ／Ｌ生根率达１００％，根系发达，适宜的温度、较高的湿度和控制杂菌是提高组培苗移栽成活率的重要因素。
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| 关 键 词： | 切花菊 组织培养 移栽 快速繁殖 |
Rapid propagation of chrysanthemum cutting flower by tissue culture |
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| CHENG Guang you ,WEI Li wen.Rapid propagation of chrysanthemum cutting flower by tissue culture[J].Journal of Agricultural Science Yanbian University,2000,22(3):203-206. |
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| Authors: | CHENG Guang you WEI Li wen |
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| Institution: | CHENG Guang you 1,WEI Li wen 2 |
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| Abstract: | Axillary bud, stem, alabastrum, leaf, and petiole of chrysanthemum cutting flower were cultured in MS medium which include 6-BA, IBA or NAA. Stem is the best explant to form adventitious buds, and MS 6-BA1.0 mg/L NAA0.1mg/L is suitable medium to induce adventitious buds. 1/2MS IBA1.0 mg/L is the best medium to induce adventitious roots. There will be higher survival rate of tube seedlings after transplanting when keeping suitable temperature, higher humidity, and killing germs. |
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| Keywords: | Chrysanthemum cutting flower tissue culture transplant |
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