A filter-assisted culture method for isolation of Treponema
spp. from bovine digital dermatitis and their identification by MALDI-TOF
MS |
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| Authors: | Isabelle Brodard Maher Alsaaod Corinne Gurtner Joerg Jores Adrian Steiner Peter Kuhnert |
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| Affiliation: | Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland;Clinic for Ruminants, Vetsuisse Faculty, University of Bern, Bern, Switzerland;Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland |
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| Abstract: | Digital dermatitis (DD) is a major infectious foot disease of cattle worldwide. Some DD stages are associated with lameness, and the disease has significant economic and animal welfare consequences. The pathogenesis of the disease is not yet fully understood, but Treponema spp. have been associated consistently with clinical cases. Isolation of these fastidious bacteria is difficult and cumbersome. We describe an improved method enabling the culturing of the 3 Treponema spp. (T. pedis, T. phagedenis, and T. medium) from bovine foot specimens derived from DD lesions, using a combination of membrane filtering and subsequent growth on selective agar media. The entire procedure from sampling to verification of individual Treponema spp. takes up to 24 d. In addition, we established a MALDI-TOF MS–based identification method to be applied for confirmation of the different Treponema spp. This scheme provides an unambiguous, simple, and straightforward identification procedure for DD-associated Treponema spp. |
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| Keywords: | cattle culture digital dermatitis MALDI-TOF MS non-healing lesion Treponema spp |
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