舞毒蛾脑和咽下神经节的形态及其三维模型构建

为探究舞毒蛾控制和分泌滞育激素的器官，于室内对舞毒蛾幼虫的中枢神经系统进行显微解剖，对其中枢神经系统和咽下神经节进行观察，并测量舞毒蛾1~5龄幼虫咽下神经节长度、宽度和面积，运用共聚焦激光扫描显微镜对脑和咽下神经节玻片逐层扫描，获取舞毒蛾幼虫脑和咽下神经节各高度的图像，将扫描后的图片描绘成1组多边图形，将其导入软件中，软件将多边图形叠加后构建其三维模型。结果显示，舞毒蛾幼虫的中枢神经系统包括脑、咽下神经节、3个胸部神经节和7个腹部神经节；舞毒蛾不同龄期幼虫的咽下神经节在外观形态上无较大差异；随着龄期增长，舞毒蛾咽下神经节的长度、宽度以及面积也不断增加，其中4龄幼虫咽下神经节的长度、宽度以及面积的增长速度最快，而5龄幼虫咽下神经节的长度、宽度以及面积的增长速度略微下降，1~3龄幼虫咽下神经节的长度、宽度以及面积的增长速度大致相同，均小于4龄和5龄幼虫。通过测量外部形态结构重建了舞毒蛾脑和咽下神经节的三维模型。

Morphology and three-dimensional modeling of the brain and subpharyngeal ganglion in gypsy moth Lymantria dispar

The central nervous system of gypsy moth Lymantria dispar larvae was microscopically dissected to investigate the organs responsible for regulating and secreting diapause hormones. Measurements of length, width, and area were taken for the pharyngeal ganglion, central nervous system of L. dispar larvae ranging from 1st instar to 5th instar. Data was obtained by scanning brain and subpharyngeal ganglion slides using a confocal laser scanning microscope. Confocal laser scanning microscopy was used to perform a layer-by-layer scan of brain and subesophageal ganglion sections. Images at various heights of the brain and subesophageal ganglion of the cotton bollworm larvae were captured. The scanned images were represented as a series of polygons, imported into software, and superimposed to construct their three-dimensional models. The findings demonstrated that the central nervous system in L. dispar larvae consisted of the brain, pharyngeal ganglion, three thoracic ganglions, and seven abdominal ganglions. The subpharyngeal ganglion across several L. dispar instar larvae did not significantly vary from one another. The pharyngeal ganglion of L. dispar was growing in length, breadth, and area as larvae progressed. The 4th instar larva exhibited the fastest development rate in terms of length,width, and area, while the 5th instar larva showed a modest drop in growth rate in these dimensions. The pharyngeal ganglion length, width and area of 1st-3th instar larvae grew at a relatively constant pace, which was lower than those of the 4th and 5th instar larvae. A three-dimensional model of the pharyngeal ganglion and brain of L. dispar was created by assessing the exterior morphological structure.