| 鸡传染性法氏囊病毒基因组A节段编码前体多聚蛋白的遗传踪迹分析 |
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| 引用本文： | 侯仲杰,王靖飞,付朝阳,高宏雷,高玉龙,祁小乐,郭莹莹,王笑梅.鸡传染性法氏囊病毒基因组A节段编码前体多聚蛋白的遗传踪迹分析[J].中国预防兽医学报,2012,34(1):28-32. |
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| 作者姓名： | 侯仲杰 王靖飞 付朝阳 高宏雷 高玉龙 祁小乐 郭莹莹 王笑梅 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 现代农业肉鸡产业技术体系建设 |
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| 摘 要： | 为研究鸡传染性法氏囊病毒(IBDV)毒力变异的分子机理,本实验采用进化踪迹分析方法对GenBank中登录的28个毒力不同的IBDV参考病毒株的基因组A节段编码的前体多聚蛋白(NH2-pVP2-VP4-VP3-COOH)推导的氨基酸序列进行分析。结果表明超强IBDV与标准IBDV的差异位点集中在第299位、451位、680位、715位和751位氨基酸上;致弱IBDV与标准IBDV的差异位点在第242位、253位、330位和981位氨基酸。该结果为进一步研究IBDV的毒力变异奠定基础。
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| 关 键 词： | 鸡传染性法氏囊病毒 前体多聚蛋白 毒力变异 进化踪迹分析 |
Evolutionary trace analysis of the amino sequences of precursor polyprotein encoded by the genome segment A of infectious bursal disease virus |
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| HOU Zhong-jie
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WANG Jing-fei
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FU Chao-yang
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GAO Hong-lei
,
GAO Yu-long
,
QI Xiao-le
,
GUO Ying-ying
,
WANG Xiao-mei.Evolutionary trace analysis of the amino sequences of precursor polyprotein encoded by the genome segment A of infectious bursal disease virus[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(1):28-32. |
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| Authors: | HOU Zhong-jie WANG Jing-fei FU Chao-yang GAO Hong-lei GAO Yu-long QI Xiao-le GUO Ying-ying WANG Xiao-mei |
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| Institution: | (State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To understand molecular basis for virulence divergence of infectious bursal disease virus(IBDV),a total of 28 amino sequences of precursor polyprotein(NH2-pVP2prot-VP3-COOH) encoded by genome segment A of reference IBDV strains in GenBank were collected and analyzed using the method of evolutionary trace analysis.The results found that the amino acids of precursor polyprotein of very virulent IBDV differ from those of classic IBDV at the positions of aa299,aa451,aa680,aa715 and aa751,while those of attenuated IBDV differ from those of classic IBDV at the positions of aa242,aa253,aa330 and aa981.These results might facilitate further the studies on IBDV virulence divergence. |
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| Keywords: | infectious bursal disease virus precursor polyprotein virulence divergence evolutionary trace analysis |
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