| 猪瘟病毒E2蛋白与猪RACK1相互作用的鉴定 |
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| 引用本文： | 凌理军,李素,董泓,贺番,冯烁,廖亚金,申梁,孙元,翁长江,仇华吉.猪瘟病毒E2蛋白与猪RACK1相互作用的鉴定[J].中国预防兽医学报,2012,34(4):279-282. |
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| 作者姓名： | 凌理军 李素 董泓 贺番 冯烁 廖亚金 申梁 孙元 翁长江 仇华吉 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪传染病研究室,黑龙江哈尔滨,150001 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项 |
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| 摘 要： | 为筛选和鉴定与猪瘟病毒(CSFV)E2蛋白相互作用的猪宿主蛋白,我们采用酵母双杂交方法筛选猪肺泡巨噬细胞表达文库得到与CSFV E2蛋白相互作用的宿主细胞RACK1蛋白,经共转化试验和GST pull-down试验进一步证实两者可以特异性结合,并且共聚焦试验表明两者共定位于细胞的细胞浆。本研究显示E2蛋白与宿主细胞RACK1蛋白存在相互作用,RACK1在CSFV感染过程中所发挥的功能有待进一步研究。
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| 关 键 词： | 猪瘟病毒 猪巨噬细胞表达文库 酵母双杂交 E2蛋白 RACK1 相互作用 |
ldentification of the interaction between classical swine fever virus E2 protein and porcine RACK1 |
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| LING Li-jun
,
LI Su
,
DONG Hong
,
HE Fan
,
FENG Shuo
,
LIAO Ya-jin
,
SHEN Liang
,
SUN Yuan
,
WENG Chang-jiang
,
QIU Hua-ji.ldentification of the interaction between classical swine fever virus E2 protein and porcine RACK1[J].Chinese Journal of Preventive Veterinary Medicine,2012,34(4):279-282. |
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| Authors: | LING Li-jun LI Su DONG Hong HE Fan FENG Shuo LIAO Ya-jin SHEN Liang SUN Yuan WENG Chang-jiang QIU Hua-ji |
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| Institution: | (Division of Swine Infectious Diseases,State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
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| Abstract: | To screen and identify the porcine proteins interacted with classical swine fever virus(CSFV) E2 protein,a potential interacting partner of RACK1 was screened with CSFV E2 as bait protein by yeast two-hybrid from porcine alveolar macrophage expression library.The interaction of the two proteins was further confirmed by co-transformation,GST pull-down and laser confocal assays.These results proved that CSFV E2 interacted with RACK1. |
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| Keywords: | classical swine fever virus porcine alveolar macrophage expression library yeast-two-hybrid E2 protein RACK1 interaction |
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