木霉对草坪褐斑病的拮抗效果及耐药性

通过木霉属5菌株与大连高尔夫球场上草坪褐斑病菌的对峙培养试验,研究结果表明:哈茨木霉Thar1菌株、哈茨木霉Thar2菌株、深绿木霉Tat菌株、钩状木霉Tha菌株及桔绿木霉Tci菌株对病原菌的抑制率为:66%、73%、71%、77%、55%,而相对抑菌效果分别为2.06、3.94、2.35、3.54、2.27,可以作为草坪褐斑病菌的生防菌加以利用。在这5株木霉中以哈茨木霉Thar2对草坪褐斑病菌的拮抗作用最强。观察结果表明,哈茨木霉Thar2对草坪褐斑病菌的拮抗机制主要表现为生长竞争、重寄生及产生抗菌物质。

Antagonistic Mechanism of Antagonistic Trichoderma Isolates against Turfgrass Rhizoctonia solani and Their Resistance to Funficide

The antagonistic experiment between Trichoderma spp.and Rhizoctonia solani, the turfgrass Rhizoctonia blight pathogen occurring in Dalian golf courses,shows that T.harzianum 1,T.harzianum 2,T.hamatum,T.citrinoviride exhibited evident inhibiting effect against the soil-borne pathogens.They are of potential biocontrol agents of the Rhizoctonia solani. Among the tested Trichoderma isolates,T.harzianum 2 showed the strongest antagonistic effect.The examination of antagonistic mechanism showed that Trichoderma spp.hyphae growed quickly, the hyphae could adhere to,coil around the hyphae of the pathogen.Trichoderma spp.could also secret antibiotic substances to cause the pathogen hyphal wall break,hyphae fragment,plasma condense and rupture.In order to improve the effect of multiple prevention and cure,this test do some indoors determination of the restraining ability of fore Trichoderma strains with fore funficides which are often used to control.