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小叶锦鸡儿总黄酮的提取方法比较与定性检验
引用本文:牛宇,牛伟,蒙秋霞.小叶锦鸡儿总黄酮的提取方法比较与定性检验[J].农产品加工.学刊,2012(7):21-23,28.
作者姓名:牛宇  牛伟  蒙秋霞
作者单位:1. 山西省农业科学院农业资源与经济研究所,山西太原,030006
2. 山西省农业科学院,山西太原,030006
3. 山西省农业科学院农业环境与资源研究所,山西太原,030006
基金项目:山西省回国留学人员科研资助项目,山西省农业科学院院级项目
摘    要:利用乙醇提取法、热水提取法和碱提酸沉法,对小叶锦鸡儿不同部位的总黄酮提取进行比较。结果表明,利用体积分数70%的乙醇提取是一种比较好的提取方法。以乙醇作溶剂来提取小叶锦鸡儿不同部位的总黄酮,其中花中黄酮得率最高,达到2.16%,叶中次之,茎皮部最低。对小叶锦鸡儿黄酮类化合物作的定性检验结果表明,小叶锦鸡儿叶和花中可能含有黄酮类、黄酮醇类、异黄酮类、查耳酮和二氢黄酮类,茎皮部可能含有黄酮类、黄酮醇类、异黄酮类和查耳酮。

关 键 词:小叶锦鸡儿  总黄酮  提取

Extraction and Qualitative Detection of Total Flavonoids in Caragana microphylla Lam.
NIU Yu , NIU Wei , MENG Qiu-xia.Extraction and Qualitative Detection of Total Flavonoids in Caragana microphylla Lam.[J].Nongchanpin Jlagong.Xuekan,2012(7):21-23,28.
Authors:NIU Yu  NIU Wei  MENG Qiu-xia
Institution:1.Institute of Agricultural Resources and Economy,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030006,China;2.Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030006,China;3.Institute of Agricultural Environment and Resources,Shanxi Academy of Agricultural Sciences,Taiyuan,Shanxi 030006,China)
Abstract:Different extraction methods of total flavonoids in flower,stem cortex and leaf of Caragana microphylla using aqueous ethanol solution,hot water and alkali/acidic aqueous solutions are compared.The results show that the yield of total flavonoids is the highest when aqueous ethanol solution is used for extraction.In the extraction with aqueous ethanol solution,the highest yield of total flavonoids is obtained from flowers,which reached 2.16%,and followed by leaves and the cortex of stems.Furthermore,the qualitative detection of total flavonoids in C.microphylla shows that its leaves and flowers might contain flavones,flavonols,isoflavones,chalcones and dihydroflavones,and the cortex of its stems might contain flavones,flavonols,isoflavones and chalcones.
Keywords:Caragana microphylla  total flavonoids  extraction
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