Diversity of Citrusdepressa Hayata (Shiikuwasha) revealed by DNA analysis |
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| Authors: | Masashi Yamamoto Akiko Takakura Aika Tanabe Sayuri Teramoto Masayuki Kita |
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| Affiliation: | 1.Faculty of Agriculture,Kagoshima University,Kagoshima,Japan;2.Faculty of Agriculture,University of the Ryukyus,Nakagami-gun,Japan;3.Okitsu Citrus Research Station,NARO Institute of Fruit Tree Science,Okitsunakacho, Shimizu,Japan;4.JICA Ethiopia Office,Addis Ababa,Ethiopia |
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| Abstract: | Citrus depressa Hayata is an indigenous mandarin species on the Ryukyu Islands located in the subtropical region of Japan. We deduced its phylogenetic relationships by evaluating accessions grown on various Ryukyu Islands via cleaved amplified polymorphic sequence analysis of cpDNA and sequence-related amplified polymorphism (SRAP). The cpDNA results indicated that C. depressa could be classified into two types. SRAP revealed patterns of diversity within C. depressa consistent with our cpDNA results. These results indicate that maternal origin may influence or is correlated with the constitution of the nuclear genome of C. depressa. Another Japanese mandarin species, Citrus tachibana (Makino) Tanaka was distinguished from C. depressa by SRAP markers. Moreover, both C. depressa and C. tachibana could be distinguished from other Citrus species. Our results suggest that Japanese mandarin possesses a characteristic genome with the genus Citrus. |
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