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微囊化蜡样芽孢杆菌的喷雾干燥工艺优化
引用本文:黄乐天 曹 丁 黄魁英等. 微囊化蜡样芽孢杆菌的喷雾干燥工艺优化[J]. 安徽农业科学, 2014, 0(20): 6546-6548
作者姓名:黄乐天 曹 丁 黄魁英等
作者单位:[1]广东省广州市微生物研究所,广东广州510000 [2]广东省广州市华元生物科技有限公司,广东广州510000 [3]广东省广州市华琪生物有限公司,广东广州510000
基金项目:广州市民生科技重大专项(201300000067;201300000068).
摘    要:[目的]提高蜡样芽孢杆菌在压力喷雾干燥过程中的存活率,优化喷雾干燥工艺。[方法]以微囊化的蜡样芽孢杆菌为材料,考察进风温度、进料流量、料液比、喷枪孔径等对喷雾细胞存活数的影响。[结果]当进风温度为140℃,进料流量为1.5 t/h,料液比为1∶5,喷枪孔径为2.5 mm时,蜡样芽孢杆菌的存活率最高,达81%。[结论]该研究确定了蜡样芽孢杆菌的最佳喷雾干燥工艺条件。

关 键 词:蜡样芽孢杆菌  压力喷雾干燥  工艺优化

Optimization of Spray-Drying Process Conditions for Microencapsulated Bacillus cereus
Affiliation:HUANG Le-tian et al ( Guangzhou Institute of Microbiology, Guangzhou, Guangdong 510000)
Abstract:[ Objective ] The spray-drying conditions were optimized for increasing the viability of microencapsulated Bacillus cereus by pressure spray-drying. [ Method ] A experimental design was constructed with air inlet temperature, feed flow rate, nozzle diameter and solid-liquid ratio as the independent variables and total viable counts as the dependent variables. [ Result ] The optimum spray-drying conditions for the production of maximally viable microencapstdated Bacillus cereus were air inlet temperature 140 ℃, feed flow rate 1.5 t/h, nozzle diameter 2.5mm, solid-liquid ratio 1 : 5,which the survival rate of Bacillus cereus was 81%. [ Conclusion] The optimum spray-drying conditions for the production of maxi- mally viable microencapsulated Bacillus cereus was determined.
Keywords:Bacillus cereus  Pressure spray drying  Optimization
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