响应面法优化琼胶酶发酵培养基 |
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引用本文: | 修,爽,祖国仁.响应面法优化琼胶酶发酵培养基[J].安徽农业科学,2014(23):7729-7731. |
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作者姓名: | 修 爽 祖国仁 |
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作者单位: | 大连工业大学生物工程学院,辽宁大连116034 |
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摘 要: | 目的]优化海洋细菌Vibrio agarivorans-1产琼胶酶液体发酵培养基的最佳组分。方法]首先通过单因素试验筛选出3个重要因子(琼脂、NaCl和酵母膏质量浓度),在单因素的基础上,通过Box-Behnken试验设计和响应面分析法确定了3个因子的最优发酵条件。结果]最佳营养组分为:琼脂粉质量浓度为3.9 g/L,NaCl质量浓度为45.2 g/L,酵母膏质量浓度为12.9 g/L,此条件下酶活力达到7.589 U/g,比优化前提高了3.35倍。结论]该研究对提高海洋细菌Vibrio agarivorans-1产琼胶酶具有重要意义。
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关 键 词: | 海洋细菌Vibrio agarivorans- 琼胶酶 响应面 |
Agarase Optimization of Fermentation Medium by Response Surface Method |
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Institution: | XIU Shuang et al (School of Biological Engineering, Dalian Polytechnic University, Dalian, Liaoning 116034) |
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Abstract: | Objective] The components of liquid fermentation medium of marine microorganisms Vibrio agarivorans-1 producing agarase were optimized. Method ] Three important factors: the concentration of agar, NaC1 and yeast extract in liquid fermentation medium, were screened by single factor experiment. The optimum fermentation conditions were determined by three factors Box-Behnken experimental design and re- sponse surface analysis based on single factor experiment. Result] The experimental result indicated that the activity of agarase reached at 7. 602 U/g as the concentration of agar, NaCI and yeast extract was 3.9 g/L, 45.2 g/L and 12.9 g/L, respectively, which was 3.35 times higher than the previous. Conclusion] The research has significance for enhancing marine microorganisms Vibrio agarivorans-1 producing agarase. |
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Keywords: | Marine bacterium Vibrio agarivorans-1 Agarase Response surface |
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