| 以LacZ为报告基因探讨促进基因表达及基因免疫应答物质的筛选 |
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| 引用本文： | 陈创夫,余兴龙,马正海,李红卫,李作生,吕宗吉,涂长春,殷震.以LacZ为报告基因探讨促进基因表达及基因免疫应答物质的筛选[J].塔里木农垦大学学报,2000(3). |
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| 作者姓名： | 陈创夫 余兴龙 马正海 李红卫 李作生 吕宗吉 涂长春 殷震 |
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| 作者单位： | 塔里木农垦大学动物科技学院!新疆阿拉尔843300(陈创夫，马正海)，解放军军需大学!吉林长春130062(余兴龙，李红卫，李作生，涂长春)，佛山科技大学!广东佛山528000(吕宗吉)，解放军军需大学!吉林(殷震) |
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| 基金项目： | “九五”国家科技攻关项目 !(C96-0 4-0 1) |
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| 摘 要： | 应用分子生物学技术构建了LacZ报告基因的pcDLacZ真核表达载体 ,并对多种促进基因表达的物质进行了筛选。应用筛选的促进基因表达物质对pcDST基因疫苗的免疫增强效果进行了观察。结果 :构建的LacZ报告基因的pcDLacZ真核表达载体 ,肌注后 1天就有表达 ,到第 7天达到高峰。通过对多种促进基因表达的物质进行筛选证明 ,4.5 %斯苯、1 %甘油、2 5 %蔗糖有明显促进基因的表达作用。 4.5 %斯苯、1 %甘油能促进pcDST猪瘟基因疫苗的免疫效果。表明 :4.5 %斯苯、1 %甘油可做为基因疫苗的免疫佐剂使用
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| 关 键 词： | LacZ报告基因 基因疫苗 免疫 |
Using LacZ Report Gene Selected Matter of Advanced Gene Expression and Gene Immune Response |
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| Chen Chuangfu Yu Xinglong Ma Zhenghai Li Hongwei,Li Zhuosheng Lu Zhongji Tu Changchun Yin Zhen.Using LacZ Report Gene Selected Matter of Advanced Gene Expression and Gene Immune Response[J].Journal of Tarim University of Agricultural Reclamation,2000(3). |
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| Authors: | Chen Chuangfu Yu Xinglong Ma Zhenghai Li Hongwei Li Zhuosheng Lu Zhongji Tu Changchun Yin Zhen |
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| Institution: | Chen Chuangfu 1 Yu Xinglong 2 Ma Zhenghai 1 Li Hongwei 2 Li Zhuosheng 2 Lu Zhongji 3 Tu Changchun 2 Yin Zhen 2 |
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| Abstract: | The eukaryotic expression plasmid, pcDLacZ, was constructed and several kinds of compound which improve the expression and immune response of DNA vaccines in vivo were screened. The results demonstrated that The eukaryotic expression plasmid, pcDLacZ, was constructed by inserting LacZ gene into pcDNA3 as a report gene; 4.5% span and 1% glycerol could improve expression and immune response in vivo by DNA vaccines. 4.5% span and 1% glycerol can be used as adjuvant to enhance the immune response of DNA vaccine |
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| Keywords: | LacZ report gene DNA vaccine immune |
| 本文献已被 CNKI 等数据库收录！ |
