百宜黑鸡与兴义矮脚鸡线粒体DNA 控制区遗传多样性分析

采用PCR和直接测序的方法测定百宜黑鸡和兴义矮脚鸡线粒体DNA控制区全序列,比较分析两种贵州地方鸡种的遗传变异。结果表明,百宜黑鸡、兴义矮脚鸡mtDNA控制区全序列长均为1231 bp;共发现26个变异位点(不包含种内变异位点),占分析位点总数的2.11%,其中12个转换,14个颠换;百宜黑鸡mtDNA控制区的A、T、G、C碱基含量分别为27.096%、33.628%、13.117%、26.159%,矮脚鸡的A、T、G、C碱基含量分别是A26.916%、T33.409%、G13.387%、C26.288%,t检验结果显示,两种鸡mtDNA控制区的A和T含量差异显著。百宜黑鸡和兴义矮脚鸡的遗传距离是0.0379,根据分子钟计算,二者约在190万年前分歧进化。

Genetic Diversity in Baiyi Black Chicken and Xingyi Bantam by Analyzing the mtDNA Control Region

PCR and direct sequencing methods were used to analyze the entire mtDNA D-loop in Baiyi Black chicken and Xingyi Bantam. The D-loop of Baiyi Black chicken and Xingyi Bantam was 1231 bp,there were 26 variational sites, including 12 transitions and 14 transversions. The nucleotide composition of Baiyi Black chicken was A 27.096%, T 33.628%, G 13.117%, C 26.159%, and A 26.916%, T 33.409%, G 13.387%, C 26.288% of Xingyi Bantam. The genetic distance between two partridges was 0.0379. According to the calibrated rates of molecular evolution, they speciated about 1.9 million years ago.