Cross-bred Cattle Protected against Hyalomma anatolicum anatolicum by Larval Antigens Purified by Immunoaffinity Chromatography |
| |
Authors: | Ghosh S Khan MH Ahmed N |
| |
Institution: | (1) Entomology Laboratory, Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243 122 Uttar Pradesh, India;(2) Protein Chemistry Laboratory, Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar, 243 122 Uttar Pradesh, India |
| |
Abstract: | Antigens from larvae of Hyalomma anatolicum anatolicum were extracted and purified by immunoaffinity chromatography using immunoglobulin ligands from cross-bred animals immunized with soluble larval antigen. Affinity-purified antigen (Aff-TLE) and a total larval extract (TLE) were used to immunize cross-bred (Bos indicus×Bos taurus) cattle. The group immunized with Aff-TLE rejected 71.6% of larvae and 77.3% of nymphs. However, the rejection percentages were lower in the TLE-immunized group. No significant changes in the feeding period, moulting percentages or moulting period of engorged larvae and nymphs were recorded. There was, however, a significant decrease in the number of resultant nymphs p<0.01) and adults (p<0.01) in the ticks fed on the Aff-TLE-immunized group. The Aff-TLE antigen was 93.3% purified. SDS-PAGE analysis identified a 39 kDa protein, reported for the first time, as the antigen responsible for the induction of resistance in the host. |
| |
Keywords: | antigen cattle Hyalomma anatolicum anatolicum immunization immunoaffinity larvae protection 39 kDa protein |
本文献已被 SpringerLink 等数据库收录! |
|