试验性趾叶炎小鼠肠组织中P物质的表达

目的]为动物趾叶炎的预防和治疗提供免疫学基础。方法]对昆明种小白鼠注射二磷酸组织胺制备趾叶炎小鼠病理模型,以注射生理盐水为对照。分别于给药后2、14、24、48、72 h及15、30 d取小鼠3~5 mm长的小肠组织块,检测肠组织中炎性细胞、杯状细胞、肥大细胞及其脱颗粒数、P物质表达水平的动态变化。结果]趾叶炎小鼠的肠腺结缔组织内及其绒毛上P物质表达异常丰富,且P物质呈单根或片状走行,并与肥大细胞串联及相邻排列,随给药时间的延长,P物质表达逐渐增强,肥大细胞及其脱颗粒数也急剧增多 炎性细胞、杯状细胞、腺体数量与肥大细胞的变化趋势一致 给药72 h后,血管内壁出现裂隙,肠粘膜出现水肿。结论]肥大细胞参与小鼠趾叶炎的发病过程,并影响小鼠肠粘膜的通透性。

Expression of Substance P in Intestinal Tissue of Experimental Mice with Digital Lobule Inflammation

Objective] The aim was to provide the immunological basis for prevention and therapy of digital lobule inflammation.Method] The Kunming white mice were injected with histamine diphosphate to prepare pathological model with digital lobule inflammation,with the treatment of injecting normal saline as CK.After 2,14,24,48,72 h and 15,30 d of the administration,the 3-5 mm intestinal tissue mass were taken to detect the dynamic changes of inflammatory cells,goblet cells,mast cells and their degranulation number and the expression level of substance P.Result] The expression of substance P in intestinal gland connective tissue and on its villi of mice with digital lobule inflammation was abnormally rich.And substance P showed single or lamellar distribution and connected and neighboring arranged with mast cell.With the prolonging of administration time,the expression of substance P gradually increased,and the mast cells and their degranulation number dramatically increased.The changing trend of inflammatory cells,goblet cells and gland number was similar with that of mast cell.After 72 h of administration,the blood vessel wall appeared cracks and intestinal mucosa appeared edema.Conclusion] Mast cells participated the pathogenic process of digital lobule inflammation of mice and influenced the permeability of intestinal mucosa.