Qualitative and quantitative strategies of thermal adaptation of grass carp (Ctenopharyngodon idella) cytoplasmic malate dehydrogenases |
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Authors: | Jen-jen Lin Shirley MacLeod Ching-ming Kuo |
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Institution: | (1) Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei, Taiwan 10647 ROC;(2) Hopkins Marine Station, Stanford University, 93950-3094 Pacific Grove, CA, USA |
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Abstract: | Malate dehydrogenase isozymes of grass carp,Ctenopharyngodon idella, were identified by mitochondrial preparation and thermal denaturation. The structural and kinetic characteristics of chromatographically separated thermostable and thermolabile cMDHs were different in (1) half-life at 42°C, 10 min and 24 h, respectively, (2) optimal substrate, oxaloacetate and malate, concentrations, and (3) the apparent Michaelis-Menten constants of NADH and oxaloacetate.Total MDH activity in white muscle of 11°C-acclimated fish was about twice that of the 30°C-acclimated group. In addition, the ratio of the thermostable to thermolabile cMDH activity in white muscle of 30°C-acclimated fish was significantly higher than that of 11°C-acclimated fish. These results suggest that temperature acclimation can induce temperature compensation in MDH activity and differential expression of thermostable and thermolabile cMDH isozymes in freshwater fish. |
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Keywords: | temperature malate dehydrogenase acclimation adaptation grass carp Ctenopharyngodon idella teleost fish thermostability isozyme kinetics |
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