| 耐盐碱基因eIF1A对玉米遗传转化研究 |
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| 引用本文： | 孟令聪,张朝澍,孟宪玉,王乾坤,曾兴,王振华,邸宏.耐盐碱基因eIF1A对玉米遗传转化研究[J].玉米科学,2014,22(2):41-45,52. |
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| 作者姓名： | 孟令聪 张朝澍 孟宪玉 王乾坤 曾兴 王振华 邸宏 |
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| 作者单位： | 东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;东北农业大学农学院, 哈尔滨 150030;黑龙江八一农垦大学寒地作物种质改良与栽培重点实验室, 黑龙江 大庆 163319 |
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| 基金项目： | 黑龙江省寒地作物种质改良与栽培重点实验开放课题项目（CGIC201202） |
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| 摘 要： | 以来自柽柳的耐盐碱基因eIF1A为研究对象，构建其单子叶植物表达载体，并利用农杆菌介导法转化极早熟玉米自交系加2和M1的胚性愈伤组织，创制转eIF1A基因玉米新材料，为耐盐碱转基因玉米育种提供技术和材料支持。结果表明，成功构建eIF1A基因的单子叶植物表达载体pCAMBIA3300-Ubi-eIF1A，共获得33株除草剂抗性植株，其中，12株目的基因和筛选标记基因PCR呈阳性反应，RT-PCR检测表明，目的基因正常转录表达。
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| 关 键 词： | 玉米 eIF1A基因 单子叶植物表达载体 遗传转化 |
| 收稿时间： | 2013/5/27 0:00:00 |
Transformation in Maize of Salt-tolerant Gene eIF1A |
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| MENG Ling-cong,ZHANG Chao-shu,MENG Xian-yu,WANG Qian-kun,ZENG Xing,WANG Zhen-hua and DI Hong.Transformation in Maize of Salt-tolerant Gene eIF1A[J].Journal of Maize Sciences,2014,22(2):41-45,52. |
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| Authors: | MENG Ling-cong ZHANG Chao-shu MENG Xian-yu WANG Qian-kun ZENG Xing WANG Zhen-hua and DI Hong |
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| Institution: | Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Northeast Agricultural University, Harbin 150030;Key Laboratory of Crop Germplasm Improvement and Cultivation in Cold Regions, Heilongjiang Bayi Agricultural University, Daqing 163319, China |
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| Abstract: | The monocot expression vector of salt-tolerant gene eIF1A cloned from Tamarix were constructed in this study, and introduced it into embryogenic callus of two very early maturing maize lines M1 and Jia2 by Agrobacterium-mediated method. The aim of this study was to create the new maize materials with eIF1A gene, for maize breeding. The results showed that monocot expression vector of eIF1A gene was constructed successfully named pCAMBIA3300-Ubi-eIF1A. There were 33 herbicide resistant plants and 12 of them were positive in PCR and RT-PCR analysis. |
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| Keywords: | Maize eIF1A gene Monocot expression vector Genetic transformation |
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