乌桕根皮醇提物萃取部位抗猪大肠杆菌活性分析

[目的]探讨乌桕根皮醇提物各部位对猪大肠杆菌4种血清型的抗菌活性。[方法]用95%乙醇80℃回流提取乌桕根皮,将醇提液浓缩后依次用石油醚、乙酸乙酯和正丁醇萃取,最后得到萃余的水相,测试各部位的抗菌活性。[结果]醇提物的石油醚部位、正丁醇部位和水部位没有抑制作用,乙酸乙酯部位对血清型K88F、41和987P具有抑制效果,而对血清型K99无效。因此,用中药材防治猪大肠杆菌疾病时,应该考虑到血清型差异。[结论]乌桕根皮提取物可以在一定程度上防治大肠杆菌引起的猪病。

Antibacterial Activity of Fractions of Ethanol Extracts from Root Bark of Sapium sebiferum against Escherichia coli from Swine

[Objective] The antibacterial activity of fractions of ethanol extracts from root bark of S.sebiferum was evaluated against 4 serotype of E.coli from swine.[Method] Root bark of Sapium sebiferum was extracted by 95% ethanol,and ethanol extraction was fractioned by petroleun ether,ethyl acetate and n-Butanol.Antibacterial activity of four fractions was tested.[Result] The fractions of petroleun ether,n-Butanol and water didn't inhibited the tested E.coli,and the fraction of ethyl acetate inhibited 3 serotypes of E.coli(K88,F41 and 987P),but didn't express activity against K99 serotype.So,when used Chinese traditional medicines to prevent and cure swine colibacillosis,it was better to choose some suitable ones according their serotype.[Conclusion] Root bark of S.sebiferum contained compounds displaying antibacterial activity against swine E.coli.