| 土壤反硝化酶活性测定方法及影响因素研究 |
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| 引用本文： | 和文祥,魏燕燕,蔡少华.土壤反硝化酶活性测定方法及影响因素研究[J].西北农林科技大学学报(社会科学版),2006,34(1):121-124. |
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| 作者姓名： | 和文祥 魏燕燕 蔡少华 |
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| 作者单位： | 西北农林科技大学,资源环境学院,陕西,杨凌,712100 |
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| 基金项目： | 中国科学院资助项目;科技部科研项目;国家自然科学基金 |
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| 摘 要： | 借助土壤酶学的基本原理,初步建立了一种底物(KNO3)最佳浓度为10 g/L,土样不需二次离心的土壤反硝化酶活性测定方法——硝态氮剩余量法,并对其影响因素进行了研究。结果表明,该测定方法简便、快速、灵敏;有机肥的加入可显著提高土壤反硝化酶的活性,有机肥和化肥的共施是最佳的培肥措施;种植作物也可提高土壤反硝化酶活性;甲苯对土壤反硝化酶活性有明显的抑制作用,且土壤肥力水平越高,抑制幅度越大。
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| 关 键 词： | 土壤反硝化酶 测定方法 有机肥 甲苯 |
| 文章编号： | 1671-9387(2006)01-0121-04 |
| 收稿时间： | 2005-05-26 |
| 修稿时间： | 2005年5月26日 |
Study on assaying method and affecting factors of soil denitrification enzyme activity |
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| HE Wen-xiang,WEI Yan-yan,CAI Shao-hua.Study on assaying method and affecting factors of soil denitrification enzyme activity[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(1):121-124. |
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| Authors: | HE Wen-xiang WEI Yan-yan CAI Shao-hua |
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| Institution: | (College of Resource and Environment,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | By means of soil enzymatic basic theory,a simple,quick and sensitive method was established to analyze soil denitrification enzyme activity(DEA) and affecting factors.The results showed that organic fertilizer could remarkably increase soil DEA.The mixing of organic fertilizer and chemical fertilizer was the best way to improve soil fertility.Plant growth also enhanced soil DEA.Toluene significantly inhibited DEA,and the inhibition grew with the increase of soil fertility. |
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| Keywords: | denitrification enzyme assayingmethod organic fertilizer toluene |
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