樱桃叶多糖的提取、鉴别及定量测定

目的]提取樱桃叶多糖,并对其进行定性鉴别和含量测定。方法]先将樱桃叶经石油醚和乙醇脱脂、脱杂后,再采用水提醇沉法提取樱桃叶粗多糖(PPP),然后以Sevag法除去粗多糖中的蛋白,并用Molish试验(α-萘酚试验)、葸酮-硫酸法试验、Fehling试验与红外光谱分析、淀粉的碘试验对其进行定性鉴定;以硫酸-蒽酮法测定樱桃叶多糖的含量。结果]经过鉴别可知,所提灰白色物质为多糖;经测定,其含量为29.7 mg/g。结论]该方法简单,灵敏度高,重现性好,结果准确。

Extraction, Identification and Content Determination of Polysaccharides from the Leaves of Prunus pseudocerasus Lindl

Objective] To study the extraction,qualitative identification and quantitative assay of polysaccharides from the leaves of Prunus pseudocerasus Lindl.(PPP),so as to provide reference for its comprehensive application.Method] Polysaccharides were extracted with the method of water exaction and alcohol precipitation after the leaves were defatted with 95% EtOH and petroleum ether.Then,the protein in PPP was removed by Sevag method.A series of experiments were used to identify the PPP,including Molish test(α-naphthol test),anthrone-sulfuric acid method test,fehling test,fourier transform infrared spectrometry(FT-IR),and iodine test.The content of PPP was determined by anthrone-sulphuric acid colrimetry method.Results] P.pseudocerasus polysaccharides were grey white in color.The content of polysaccharides in P.pseudocerasus leaves was 29.7 mg/g.Conclusion] The method is simple,sensitive,reproducible,and reliable.