烃类污染物降解菌的筛选及其生长条件研究

目的]筛选烃类污染物降解菌,研究其生长条件。方法]以石化厂附近长期被石油污染的土壤微生物为菌源,煤油作为唯一碳源,经过驯化筛选、分离出对煤油降解效果较好的菌种,并对影响菌种生长的条件参数进行了优化。结果]煤油降解菌的最佳生长条件为:温度30℃,pH 7,盐度2.5%,恒温摇床转速190 r/min。在此条件下,将菌种培养3 d后,煤油降解率达42.6%。结论]该研究为烃类污染土壤的修复提供了理论依据。

Selection of the Bacteria of Kerosene Degradation and Study on the Growth Conditions of the Bacteria

Objective] The study aimed at selecting the predominant strains of kerosene degradation and studying its best growth conditions.Method] Choosing kerosene as the only carbon source,we selected and separated the predominant strains of kerosene degradation from the contaminated soil micro-organisms near petrochemical plants,and optimized the grow conditions of the bacteria.Result] The best conditions for the bacteria growth were determined as follows,that is,temperature was 30 ℃,pH=7,salinity was 2.5%,and the rotational speed of thermostatic shake was 190 r/min.Under the conditions,the degradation rate of kerosene by the bacteria cultured for three days reached 42.6%.Conclusion] The research could provide scientific references for the restoration of polluted soil by kerosene.