| 小G蛋白Rab2基因参与小麦抗叶锈病反应的研究 |
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| 引用本文： | 杨静静,李星,李亚宁,刘大群.小G蛋白Rab2基因参与小麦抗叶锈病反应的研究[J].华北农学报,2010,25(4). |
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| 作者姓名： | 杨静静 李星 李亚宁 刘大群 |
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| 作者单位： | 河北农业大学,植物保护学院,河北省植物病虫害生物防治工程技术研究中心,河北,保定,071001 |
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| 基金项目： | 国家自然科学基金项目，河北省教育厅基金项目 |
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| 摘 要： | 为了解叶锈菌侵染小麦后小G蛋白Rab2基因的表达情况,明确其与抗病性的关系,从分子水平上探讨小麦的抗叶锈病机制。以普通六倍体小麦近等基因系TcLr1和叶锈菌小种05-22-64①和05-8-63①为试验材料,构建小麦亲和与非亲和组合,利用实时定量PCR技术对小麦小G蛋白Rab2基因的表达情况进行检测。结果表明:在小麦叶锈菌侵染过程中,Rab2基因主要在侵染的前期表达迅速,随着时间的推移表达量有所下降。非亲和叶锈菌菌株可以诱导小G蛋白Rab2基因表达量的升高,而亲和叶锈菌菌株抑制小G蛋白Rab2基因的表达。由此表明,小G蛋白可能与寄主和病原物的亲和程度有着直接的关系。
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| 关 键 词： | 小麦叶锈病 小G蛋白 Rab2基因 实时定量PCR |
Involvement of Small GTP-Binding Protein Gene Rab2 in Resistance Response of Wheat to Its Leaf Rust Disease |
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| YANG Jing-jing,LI Xing,LI Ya-ning,LIU Da-qun.Involvement of Small GTP-Binding Protein Gene Rab2 in Resistance Response of Wheat to Its Leaf Rust Disease[J].Acta Agriculturae Boreali-Sinica,2010,25(4). |
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| Authors: | YANG Jing-jing LI Xing LI Ya-ning LIU Da-qun |
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| Institution: | YANG Jing-jing,LI Xing,LI Ya-ning,LIU Da-qun (College of Plant Protection,Agricultural University of Hebei,Biological Control Center of Plant Diseases and Plant Pests of Hebei Province,Baoding 071001,China) |
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| Abstract: | Wheat leaf rust disease caused by wheat leaf rust (Puccinia triticina) is one of the most important diseases which have led to heavy losses to the wheat production in the world,and it also is a main factor influencing the wheat production in our country. The application of the disease resistant varieties and increasing host plant resistance are the most promising ways to control this disease,which based on the fully understanding of the resistant mechanisms. In recent years,the regulation channel of small G... |
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| Keywords: | Wheat leaf rust disease Small G protein Rab2 gene Real-time quantitative PCR |
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