辣椒DNA甲基化修饰酶基因的鉴定与表达特征分析

DNA甲基化与去甲基化是表观遗传修饰中一种保守的分子机制,参与植物生长发育、次生代谢和逆境胁迫响应等多种生物过程,受DNA甲基化修饰酶基因的调控.为了解DNA甲基化修饰酶基因在辣椒基因组中的特征,利用生物信息学手段鉴定出14个辣椒DNA甲基转移酶和去甲基化酶基因,并对其进行系统分析.结果表明:辣椒基因组中含10个DNA...

Identification and Expression Analysis of the DNA Methyltransferase and Demethylase Gene Families in Capsicum annuum L.

DNA methylation or demethylation is a highly conserved epigenetic modification involved in the regulation of numerous biological processes,including plant growth and development,secondary metabolism,and response to abiotic stresses,which controlled by DNA methyltransferase and demethylase genes.To fully understand the characteristics of DNA methylation modifying enzyme genes in the pepper genome,a total of 10 putative DNA methyltransferase and 4 demethylase genes were identified in pepper in the present study by using bioinformatics methods.The amino acids encoded by the genes were between 294–2037 AA.The genes were located on 10 different chromosomes,the number of exons contained in the 14 genes is between 1 and 21.The conserved motif compositions and exon-intron structures were systematically analyzed,and the results strongly supported the classification.Transcriptome data analysis proved that the expression levels of family member genes in different organs of pepper and different stages of fruit development were different.Among them,CaCMT1 and CaMET1-like showed the lowest expression in all organs,while CaDRM1-like2 and CaROS1-like2 showed the highest.The expression pattern of genes under high temperature and salt stress was analyzed by qPCR.Compared with the whole period of high temperature treatment,it was found that the expression of methylation modifying enzyme gene changed most obviously in materials treated for 3 hours,and the genes with the highest up-regulation were CaROS1-like2 and CaROS1-like1,respectively.The methylation modifying enzyme gene of the materials induced by salt stress was the most sensitive when treated for 12 hours,and the expression of 9 genes reached the peak,and the genes with the highest up-regulation were CaROS1-like2 and CaDRM1-like1.This study would provide a theoretical reference for further revealing the regulatory role of pepper DNA methylation modifying enzyme gene family in epigenetics.