Resistance to <Emphasis Type="Italic">Meloidogyne incognita</Emphasis> expresses a hypersensitive-like response in <Emphasis Type="Italic">Coffea arabica</Emphasis> |
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Authors: | Erika Valéria Saliba Albuquerque Poliene Martins Costa Ana Cristina Meneses Mendes Gomes Marcilene Santos Antonio Alves Pereira Michel Nicole Diana Fernandez Maria Fatima Grossi-de-Sa |
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Institution: | 1.Embrapa—Recursos Genéticos e Biotecnologia,Brasília,Brazil;2.CRZM, EPAMIG,Vi?osa,Brazil;3.IRD—Institut de Recherche pour le Développement,UMR-186 IRD-Cirad-UM2 “Résistance des Plantes aux Bioagresseurs”,Montpellier-Cedex 5,France;4.Graduate Program in Cellular and Molecular Biology (PPGBCM), Center of Biotechnology,Federal University of Rio Grande do Sul (UFRGS),Porto Alegre,Brazil;5.Université de Montpellier II (UM2), Systèmes Intégrés en Biologie, Agronomie, Géosciences, Hydrosciences, Environnement (SIBAGHE),Montpellier,France |
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Abstract: | Root-knot nematodes (RKN) are obligate parasite species of the genus Meloidogyne that cause great losses in Arabica coffee (Coffea arabica L.) plantations. Identification of resistant genotypes would facilitate the improvement of coffee varieties aiming at an
environmental friendly and costless nematode control. In this work, the C. arabica genotype ‘UFV 408-28’ was found to be resistant to the most destructive RKN species M. incognita. Pathogenicity assays indicated that the highly aggressive populations of M. incognita races 1, 2 and 3 were not able to successfully reproduce on ‘UFV 408-28’ roots and displayed a low gall index (GI = 2). An
average reduction of 87% reduction of the M. incognita population was observed on ‘UFV 408-28’ when compared to the susceptible cultivar ‘IAC 15’. By contrast, ‘UFV 408-28’ was
susceptible to the related species M. exigua and M. paranaensis (GI = 5 and 4, respectively). Histological observations performed on sections of UFV408-28 roots infected with M. incognita race 1 showed that nematode infection could be blocked right after penetration or during migration and establishment stages,
at 6 days, 7 days and 8 days after infection (DAI). Fluorescence and bright field microscopy observations showed that root
cells surrounding the nematodes exhibited HR-like features such as accumulation of phenolic compounds and a necrotic cell
aspect. In the susceptible ‘IAC 15’ roots, 6 DAI, feeding sites contained giant cells with a dense cytoplasm. Necrotic cells
were never observed throughout the entire infection cycle. The HR-like phenotype observed in the ‘UFV 408-28’—M. incognita interaction suggests that the coffee resistance may be mediated by a R-gene based immunity system and may therefore provide new insights for understanding the molecular basis of RKN resistance
in perennial crops. |
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