| 植物单宁酸交联明胶的条件优化及性质分析 |
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| 引用本文： | 邵士凤,赵新淮,孙红梅. 植物单宁酸交联明胶的条件优化及性质分析[J]. 农业工程学报, 2009, 0(Z1) |
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| 作者姓名： | 邵士凤 赵新淮 孙红梅 |
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| 作者单位： | 东北农业大学乳品科学教育部重点实验室;绥化学院化学系; |
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| 基金项目： | 国家高技术发展计划(863)(2006AA10Z324);;哈尔滨市科技创新人才研究专项资金项目(2006RFXXN003) |
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| 摘 要： | 该文用单宁酸作交联剂对明胶进行交联改性,通过响应面法优化反应条件并建立回归模型,并对交联前后的明胶进行十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、紫外光谱和黏度等3方面的分析测定,确认单宁酸交联对明胶所产生的交联作用,开发新食品配料交联明胶。结果显示:适宜的交联反应条件为反应温度47.5℃,时间50h,单宁酸添加量80mg/g明胶。性质分析表明,与原料明胶相比交联明胶一些性质发生变化,紫外光谱分析发现交联明胶在276nm出现新吸收峰,表明交联明胶分子中存在苯环;电泳后交联明胶的蛋白质条带色泽明显变浅,且交联度越大条带色泽越浅,此外,交联明胶溶液的黏度明显增大,说明单宁酸交联处理使交联明胶中有更多的大分子物质生成。
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| 关 键 词： | 明胶 交联 单宁酸 性质 |
Optimization of cross-linking conditions for gelatin with vegetative tannic acid and analysis for its properties |
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| Affiliation: | 1.Key Laboratory of Dairy Science of ministry of Education;Northeast Agricultural University;Harbin 150030;China;2.Department of Chemistry;Suihua University;Suihua 152061;China |
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| Abstract: | Gelatin was modified with tannic acid as a cross-linker in this study.Cross-linking conditions of gelatin were optimized by response surface methodology and corresponding regression model was thus established.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)analysis,ultraviolet absorption spectrum analysis and viscosity determination were conducted to confirm the cross-linking of gelation by tannic acid.The results showed that the optimal cross-linking conditions were as follow:reaction te... |
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| Keywords: | gelatin cross-linking tannic acid property |
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