| 小麦未熟胚离体培养的研究——愈伤组织发生及器官建成 |
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| 引用本文： | 关畅,曾寒冰,李文雄.小麦未熟胚离体培养的研究——愈伤组织发生及器官建成[J].东北农业大学学报,1993(2). |
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| 作者姓名： | 关畅 曾寒冰 李文雄 |
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| 作者单位： | 东北农学院农学系，东北农学院农学系，东北农学院农学系 哈尔滨 150030，哈尔滨 150030，哈尔滨 150030 |
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| 摘 要： | 对小麦胚离体培养过程中愈伤组织发生及器官的形态建成进行了解剖学观察。结果表明,胚龄对愈伤组织发生的起始部位有明显影响。胚的各个部位对离体培养反应的敏感程度随胚龄的增长盾片形成愈伤组织的能力而减弱,3周龄至成熟胚的盾片不能产生愈伤组织,只起分泌α-淀粉酶、转化、吸收和运转养分的作用。同时还观察到输导系统分化完善之前存在传递细胞。不定根及不定芽的分化和形成,既存在器官发生途径,也存在胚状体途径。根、芽原基形成的先后顺序并不固定。
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| 关 键 词： | 小麦 胚培养 愈伤组织 器官发生 胚状体 |
STUDIES ON WHEAT EMBRYO CULTURE IN VITRO——CALLUS INDUCTION AND ORGANOGENESIS |
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| Guan Chang Zeng Hanbing Li Wenxiong.STUDIES ON WHEAT EMBRYO CULTURE IN VITRO——CALLUS INDUCTION AND ORGANOGENESIS[J].Journal of Northeast Agricultural University,1993(2). |
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| Authors: | Guan Chang Zeng Hanbing Li Wenxiong |
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| Abstract: | The study deals with the anatomy of callus induction and organogenesis of wheat em-
bryo in vitro culture.The study results indicated that theinitial part of callus induction was af-
fected by the age of embryos used.Different parts of embryo varied in reacting to culture in
vitro.The ability of scutellum to initiate callus decreased along with the increase of embryo
age.The scutella of 3-week-aged or older embryo couldn't produce any callus but secrete α-
amylase,absorb,transform,and transfer nutrient from the edosperm.Transfer cells were ob-
served before the establishment of conducting system.The differentiation and fromation of
adventitious root and shoot could be from either organogenesis or embryoids.Formation
sequencies of root and shoot primordia were not consistent. |
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| Keywords: | wheat embryo culture callus organogenesis embryoid in vitro |
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