H7N7亚型禽流感病毒血凝素B细胞和Th细胞表位预测及初步鉴定

摘　要 　目的 预测H7N7亚型禽流感病毒血凝素(Hemagglutinin,HA)B细胞和Th细胞相关抗原表位,并对所获表位的抗原性进行初步鉴定。方法 根据禽流感流行趋势，从GeneBank下载H7N7禽流感病毒HA氨基酸序列，使用生物信息学方法，对所下载序列进行预测与分析，获得H7N7亚型禽流感病毒血凝素B细胞和Th细胞相关抗原表位。通过H7亚型禽流感病毒阳性血清，初步验证所选表位抗原性。结果 获得了5个H7N7亚型禽流感病毒候选B细胞和Th细胞表位，经过间接ELISA方法分析，其中HA165~178，HA180~193，HA241~255表序列相对保守,与流行的H7N7亚型禽流感病毒HA相应区域具有较好的一致性，同时具有与H7型禽流感病毒阳性血清抗体结合能力,预示了其成为功能表位的可能。结论 所筛选的表位具有成为H7N7亚型禽流感病毒HAB细胞和Th细胞相关抗原表位的可能，为H7N7亚型禽流感表位疫苗的研究奠定基础。

Prediction of the B cell/Th cell epitopes on HA of avian in fluenza virus (H7N7) and preliminary evaluation

To predict B/Th cell epitopes on Hemagglutinin(HA)of avian influenza virus(H7N7)and evaluate antigenicity of the candidate epitopes.The HA amino acid sequences of AIV(H7N7)which were prevalent recently,were downloaded from GeneBank.The Th/B cell epitopes were predicted and analyzed by bioinformatics methods.Then,antigenicity of the candidate epitopes was identified by AIV(H7)positive serum samples.Five B/Th cell epitopes containing HA were acquired.HA165~178,HA180~193,HA241~255epitopes which were analyzed b...