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| 扁蓿豆愈伤组织诱导和分化条件的研究 | |
| 引用本文： | 张彦艳,王照兰,刘国栋,胡卉芳,杜建材,赵丽丽.扁蓿豆愈伤组织诱导和分化条件的研究[J].中国草地学报,2009,31(4). |
| 作者姓名： | 张彦艳 王照兰 刘国栋 胡卉芳 杜建材 赵丽丽 |
| 作者单位： | 1. 甘肃农业大学草业学院,甘肃,兰州,730070;中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室,内蒙古,呼和浩特,010010 2. 中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室,内蒙古,呼和浩特,010010 3. 内蒙古赤峰市环境监测站,内蒙古,赤峰,024000 4. 内蒙古农业大学生态环境学院,内蒙古,呼和浩特,010019 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所)资助项目，国家重点基础研究发展规划(973计划)，农业部草原资源与生态重点开放实验室资助项目 |
| 摘 要： | 以扁蓿豆3个品系的下胚轴和子叶为外植体,研究不同培养基、不同激素种类和配比对愈伤组织诱导的影响,以及不同分化培养基对愈伤组织分化的影响.结果表明:下胚轴的愈伤组织诱导率普遍高于子叶;品系B1、B2最佳愈伤组织诱导培养基为MS+0.5mg/L 2,4-D+0.5mg/L 6-BA,品系B3最佳愈伤组织诱导培养基为MS+0.5mg/L 2,4-D+0.7mg/L 6-BA;适宜分化的培养基均为MS+1mg/L KT+1mg/L 6-BA. |
| 关 键 词： | 扁蓿豆 组织培养 愈伤组织诱导 激素 愈伤组织分化 |
Study on the Conditions for Callus Induction and Callus Differentiation of Melilotoides ruthenica |
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| ZHANG Yan-yan,WANG Zhao-lan,LIU Guo-dong,HU Hui-fang,DU Jian-cai,ZHAO Li-li.Study on the Conditions for Callus Induction and Callus Differentiation of Melilotoides ruthenica[J].Chinese Journal of Grassland,2009,31(4). | |
| Authors: | ZHANG Yan-yan WANG Zhao-lan LIU Guo-dong HU Hui-fang DU Jian-cai ZHAO Li-li |
| Abstract: | The cotyledon and hypocotyls of three strains of Melilotoides ruthenica were used to study the effect of different mediums, different hormones in different concentration on callus induction and callus differentiation. The results indicated that the callus induction rate with hypoeotyls was higher than cotyle- dons. The best medium for callus induction with hypocotyls of strain B1 and B2 was MS+ 0.5mg/L 2,4- D+0.5mg/L 6-BA, the best medium for callus induction with hypoeotyls of B3 was MS+ 0.5mg/L 2,4-D +0.7mg/L 6-BA. The best medium for callus differentiation was MS+1mg/L KT+1mg/L 6-BA. |
| Keywords: | Melilotoides ruthenica Tissue culture Callus induction Exogenous hormones Callus dif- ferentiation |
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