紫心马铃薯品种黑美人组织培养技术

目的]研究紫心马铃薯品种黑美人的组织培养技术,为其种薯工厂化生产提供技术支持.方法]以紫色马铃薯品种黑美人的顶芽及不同部位腋芽为外植体,探讨0.1％ HgCl2对外植体灭菌、6-BA对初代培养、6-BA和PP333组合对增殖培养、NAA对生根、糖对结薯等过程的影响.结果]在4.0～9.0min范围内,以0.1％升汞溶液对顶芽、第2段腋芽、第3段腋芽及以下芽分别处理5、6、8 min的灭菌效果最好.在MS培养基中添加0～3.0 mg/L 6-BA,顶芽、第2段腋芽、第3段腋芽及以下芽的适宜初代培养基分别为MS＋1.0 mg/L 6-BA、MS＋1.5 mg/L 6-BA、MS＋2.0 mg/L6-BA.在MS培养基中添加0～3.0mg/L 6-BA和0～0.4 mg/L PP333,较适宜的增殖培养基为MS＋3.0 mg/L 6-BA＋0.2 mg/L PP333,最高增殖倍数为7.0.在0～0.6mg/L NAA内,较适宜的生根培养基为MS＋0.4 mg/L NAA,最高生根率达100.0％.MS培养基中添加30.0～80.0 mg/L糖,较适宜的结薯培养基为MS＋60.0 g/L糖,最高结薯率达93.3％.结论]成功构建了紫色马铃薯黑美人的再生繁殖体系,获得无菌苗及试管薯,该繁殖技术可用于其种薯工厂化生产.

Tissue culture of purple-heart potato variety Black Beauty

Objective]By using different types of explants, tissue culture of purple-heart potato variety Black Beauty was studied in order to provide technical supports for its factory production in Guangxi. Method ]The termi- nal buds, the different section axillary buds of potato variety Black Beauty were used as different explants, and MS was used as base medium. The effects of 0.1% HgCl2（Mercuric chloride ） sterilizing time on explants were investigated, as well as 6-BA on primary culture, different concentrations of 6-BA combined with PP333 on proliferation culture, NAA on rooting and sugar on tuber formation. Result]In the range of 4.0-9.0 min, the suitable sterilized time of 0.1% HgC12 for terminal buds, the 2nd section axillary bud and the 3rd section buds were 5, 6 and 8 rain, respec- tively. By adding 0-3.0 mg/L 6-BA in MS medium, the suitable primary medium for them were MS＋1.0 mg/L 6- BA, MS＋1.5 mg/L 6-BA and MS＋2.0 mg/L 6-BA, respectively. By adding combination of 0-3.0 mg/L 6-BA and 0-0.4 mg/L PP333 in MS medium,MS＋3.0 mg/L 6-BA＋0.2 mg/L PP333 was beneficial for multiplication culture, and the highest multiplication coefficient was 7.0. In the range of 0-0.6 mg/L NAA, MS medium added with 0.4 mg/L NAA was more effective for rooting culture, and the highest rooting rate was 100.0%. In the range of 30.0- 80.0 mg/L sugar, adding 60.0 g/L sugar in MS medium was appropriate for tuber formation, which resulted in 93.3% of tuberization rate. Conclusion]The regeneration propagation system of purple-heart potato variety Black Beauty was established successfully to propagate virus-free plantlets and test-tubers. This tissue culture technology could be widely used in factory production of seed-tuber in Guangxi.