ISSR分子标记对杧果实生苗父本的早期鉴定

目的]采用ISSR分子标记技术对杧果实生苗进行亲本早期鉴定,为提高杧果育种效率提供参考.方法]采用ISSR分子标记技术,筛选不同引物鉴定97-1实生苗的父本.结果]采用优化的ISSR反应体系,从18条引物中筛选获得4条特异引物UBC-848、UBC-857、UBC-864和UBC-868,其扩增条带多、稳定性强、多态性高、背景清晰.利用引物UBC-848、UBC-857、UBC-864、UBC-868分别对四川红柁、紫花柁和97-1实生苗进行扩增,获得的扩增片段长度在100～2000bp,四川红杧与97-1实生苗、紫花杧与97-1实生苗的共有特异性条带约为180和380 bp、280和380 bp、180和680 bp、680和770bp.结论]采用ISSR分子标记技术和引物UBC-848、UBC-857、UBC-864、UBC-868构建了杧果实生苗亲本鉴定体系,可鉴定出97-1实生苗的父本为四川红杧,97-1实生苗是紫花杧和四川红杧的杂交种.

Early identification of male parent of mango seedlings by ISSR molecular markers

Objective]Early identification and selection of parents of mango seedlings by ISSR molecular markers were conducted to provide references for improving mango breeding efficiency based on ISSR molecular markers. Method]Using ISSR marker technology, different primers were screened to identify male parent of 97-1 seedling. Result]According to the optimized ISSR reaction system, four specific primers were chosen from 18 primers（UBC-848, UBC-857,UBC-864 and UBC-868）, which had many amplified bands, strong stability, high polymorphism and clear backgrounds. Jyhna mango, Sichuan red-skin mango and 97-1 seedlings were amplified using primers UBC-846,UBC-857,UBC-864 and UBC-868. The length of the amplified fragments was 100-2000 bp. The size of specific bands of Sichuan red-skin mango, Jyhua mango and 97-1 seedlings was 180 and 380 bp, 280 and 380 bp, 180 and 680 bp, and 680 and 770 bp. Conclusion]Through the identification system construction of mango seedling parents using ISSR molecular markers and primers UBC-846 ,UBC- 857 ,UBC-864 and UBC-868, it was conclude that the male parent of 97-1 seedlings was Sichuan red-skin mango and 97-1 seedlings were the crosses between Jyhua mango and Sichuan red-skin mango.