| 河南省玉米穗粒腐病病原串珠镰刀菌鉴定 |
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| 引用本文： | 裴冬丽,刘春元,吴建宇.河南省玉米穗粒腐病病原串珠镰刀菌鉴定[J].玉米科学,2011,19(1):136-138. |
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| 作者姓名： | 裴冬丽 刘春元 吴建宇 |
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| 作者单位： | 1. 河南农业大学,郑州,450002;商丘师范学院生命科学系,植物与微生物互作重点实验室,河南商丘,476000
2. 河南农业大学,郑州,450002 |
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| 基金项目： | 国家高技术研究“863”计划项目(2006AA10Z1D5)、河南省科技厅基础与前沿技术项目(082300430320) |
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| 摘 要： | 河南省玉米穗粒腐病一般发病率为10%～30%,串珠镰刀菌是优势病原菌。从河南郑州感病的玉米穗轴和穗粒上分别分离出两个镰刀菌菌株。运用培养性状和形态特征综合分析的方法进行种类鉴定,并提取基因组DNA,对其内转录间隔区(ITS)序列进行PCR扩增和序列测定。结果鉴定出1个种为串珠镰刀菌。两个菌株rDNA间区ITS2可变区属I型,均能产伏马菌素。
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| 关 键 词： | 玉米 穗粒腐病 串珠镰刀菌I TS序列分析 |
| 收稿时间： | 2009/12/9 0:00:00 |
The Identification of Pathogen Fusarium verticillioides Causing Maize Ear Rot in Henan |
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| PEI Dong-li,LIU Chun-yuan,WU Jian-yu.The Identification of Pathogen Fusarium verticillioides Causing Maize Ear Rot in Henan[J].Journal of Maize Sciences,2011,19(1):136-138. |
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| Authors: | PEI Dong-li LIU Chun-yuan WU Jian-yu |
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| Institution: | PEI Dong-li1,2,LIU Chun-yuan1,WU Jian-yu1(1.Henan Agricultural University,Zhengzhou 450002,2.Key Laboratory of Plant-Microbe Interactions,Department of Life Science,Shangqiu Normal University,Shangqiu 47600,China) |
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| Abstract: | The incidence of maize ear rot is about 10% to 30% in Henan province. Fusarium verticillioides is the dominant pathogen. Two strains were separately isolated from the ear aris and ear grain of diseased maize in Zhengzhou. The strains were studied with morphological methods based on culture traits and morphological characteristics, the genomic DNA of it were extracted, the sequences of ITS were amplified and tested. The result showed that a species was identified as Fusarium verticillioides. The variable rDNA internal transcribed spacer ITS2 was type I, which could produce fumonisin. |
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| Keywords: | Maize Ear rot Fusarium verticillioides ITS sequence analysis |
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