甘蓝型油菜SnRK基因家族生物信息学分析及其与种子含油量的关系

蔗糖非发酵相关蛋白激酶(sucrose non-fermenting-1-related protein kinase,SnRK)是植物中广泛存在的丝氨酸/苏氨酸蛋白激酶,它们参与调控植物的信号传导、逆境响应和种子生长等生物学过程。为了解析甘蓝型油菜(Brassica napus L.)中SnRK基因家族的性质及其对种子含油量的影响,本研究对BnSnRK基因家族的系统进化、基因结构、蛋白质理化性质、保守基序、蛋白质二级结构、顺式作用元件和亚细胞定位预测等进行分析,并通过候选基因关联分析、单倍型分析和qRT-PCR筛选影响种子含油量的BnSnRK基因。结果显示,鉴定得到92个BnSnRK成员,分为3个亚族,分布于甘蓝型油菜19条染色体上,亚族之间蛋白质理化性质差异显著。多数基因拥有7~14个外显子;同一亚族的motif分布情况更相似。BnSnRK家族主要在细胞质中表达,蛋白质二级结构主要以α-螺旋和不规则卷曲为主。关联分析筛选出与油菜种子含油量相关的12个家族成员,基因BnaC02g10730D可能负向调控甘蓝型油菜种子含油量,基因BnaA07g12290D、BnaA10g22850D、BnaA08g18050D、BnaC04g44390D可能正向调控甘蓝型油菜种子含油量。不同环境之间种子含油量差异显著,且与含油量相关的12个成员均含有MYB、MYC和ABA响应元件,环境特异的含油量关联基因可能与植物非生物胁迫响应相关。本研究为BnSnRK基因功能验证及育种工作提供了理论参考。

Bioinformatics analysis of SnRK gene family and its relation with seed oil content of Brassica napus L.

Sucrose non-fermenting-1-related protein kinase(SnRK)is a widely existed serine/threonine protein kinase in plants,which is involved in the regulation of biological processes such as signal transduction,stress response and seed growth.In order to explore the mechamism of the SnRK gene families and its influence on the seed oil content of Brassica napus L.,BnSnRK gene family system evolution,gene structure,physical and chemical properties of protein,conservative motif,protein secondary structure,cis-element and subcellular localization prediction were analyzed,and BnSnRK genes affecting oil content of seeds were screened by candidate genes association analysis,haplotype analysis and qRT-PCR.The results showed that 92 BnSnRK members were identified and divided into three subgroups,distributed on 19 chromosomes of Brassica napus L.The physical and chemical properties of proteins were significantly different among subgroups.Most genes had 7–14 exons;the motifs of the same subfamily were more similar in distribution.The BnSnRK family was mainly expressed in the cytoplasm.12 family members related to the oil content were screened out by association analysis in rapeseed.Genes BnaC02g10730D might negatively regulate seed oil content,while genes BnaA07g12290D,BnaA10g22850D,BnaA08g18050D,and BnaC04g44390D might positively regulate seed oil content of Brassica napus L.There were significant differences in seed oil content under different environments,and 12 oil-related members all contained MYB,MYC and ABA response elements.Environment-specific oil-related genes might be related to plant abiotic stress response.This study provides a theoretical basis for BnSnRK gene functional verification and breeding.