| 利用芽孢杆菌在温室环境中控制番茄青枯病 |
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| 引用本文： | Algam A.SOAD,谢关林,李斌,郝晓娟,Jef COOSEMANS,刘波.利用芽孢杆菌在温室环境中控制番茄青枯病[J].植物病理学报,2006,36(1):80-85. |
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| 作者姓名： | Algam A.SOAD 谢关林 李斌 郝晓娟 Jef COOSEMANS 刘波 |
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| 作者单位： | 1. 浙江大学生物技术研究所, 杭州 310029;2. 比利时鲁汶大学植物保护和植物病理实验室, 鲁汶 B3001;3. 福建省农业科学院生物技术中心, 福州 350003 |
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| 摘 要： | 在离体条件下测试了81株芽孢杆菌分离物抑制番茄青枯病菌的能力,有4个菌株(B2、B5、B7和B8)显示了较好的抑菌效果,经细菌生物学性状、Biolog和16S rDNA序列分析,B2菌株鉴定为短短小芽孢杆菌(Brevibacillus brevis),B5、B7和B8鉴定为枯草芽孢杆菌(Bacillus subtilis)。在温室环境中,4个菌株都能不同程度的促进灭菌土和自然土的番茄生长,并能减轻番茄青枯病的发生。B2菌株显示了显著的抑菌作用,在灭菌土和自然土中分别降低了80.0%和87.4%的番茄青枯病发生率。
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| 关 键 词： | 生物防治 芽孢杆菌 细菌性枯萎 青枯劳尔氏菌 |
| 文章编号： | 0412-0914(2006)01-0080-06 |
| 修稿时间： | 2004年12月6日 |
Biological Control of Bacterial Wilt of Tomato by Bacillus spp. under Greenhouse Environment |
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| Algam A.SOAD,XIE Guan-lin,LI Bin,HAO Xiao-juan,Jef COOSEMANS,LIU Bo.Biological Control of Bacterial Wilt of Tomato by Bacillus spp. under Greenhouse Environment[J].Acta Phytopathologica Sinica,2006,36(1):80-85. |
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| Authors: | Algam ASOAD XIE Guan-lin LI Bin HAO Xiao-juan Jef COOSEMANS LIU Bo |
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| Institution: | 1. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China;2. Lab of Phytopathology and Plant Protection, Catholic University of Leaven, B3001, Belgium;3. Biotechnology Center, Fujian Academy of Agriculture Science, Fuzhou 350003, China |
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| Abstract: | Eighty-one isolates of Bacillus were evaluated for their ability to inhibit the growth of Ralstonia solanacearum in vitro. Four isolates (B2,B5, B7 and B8) showed inhibitory effect on bacterial growth, were identified as Brevibacillus brevis B2, Bacillus subtilis B5, Ba. subtilis B7, and Ba. subtilis B8, respectively, by bacteriological assays, Biolog test and 16S rDNA sequence analysis. Four isolates promoted plant growth and reduced the infection of tomato wilt in sterile and nonsterile soil under greenhouse conditions to a varying degree as compared with non-treated control. Strain B2 showed significantly reduction of the disease infection by 80.0% and 87.4% in sterile and nonsterile soil,respectively. |
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| Keywords: | biological control Bacillus spp bacterial wilt Ralstonia solanacearum |
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