| 藏绵羊MHC-DRB1基因第3外显子的PCR-SSCP检测及其序列分析 |
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| 引用本文： | 徐飞,成述儒,刘秀,王继卿,胡江,罗玉柱.藏绵羊MHC-DRB1基因第3外显子的PCR-SSCP检测及其序列分析[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 徐飞 成述儒 刘秀 王继卿 胡江 罗玉柱 |
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| 作者单位： | 甘肃农业大学甘肃省草食动物生物技术重点实验室/动物科学技术学院,兰州,730070 |
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| 基金项目： | 国家科技支撑计划项目(No.2009BAC53B06、No.2007BAD52B05); 国家高技术研究发展计划(863计划)项目(No.2006AA10Z196); 甘肃省农业生物技术专项(No.GNSW-2009-24); 国家农业科技成果转化资金项目(No.2010GB2G100491) |
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| 摘 要： | 为了研究藏绵羊DRB1基因第3外显子多态性,确定其等位基因数、核苷酸多态位点、变异类型和各等位基因间的遗传关系,本研究采用PCR-SSCP方法,分析了500只藏绵羊(Ovis aries)DRB1基因第3外显子多态性,并对不同等位基因进行克隆和测序。结果表明,藏绵羊DRB1基因第3外显子表现了8个等位基因,8个单倍型序列分析发现了15个核苷酸多态位点,与GenBank序列对比分析,有7个DRB1的等位基因是首次发现。8个DRB1第3外显子的单倍型序列NJ系统发育树呈2支分化趋势。3个种群藏绵羊中B均为优势等位基因,该位点PIC>0.5,为高度多态且显著偏离Hardy-Weinberg平衡状态。研究认为,藏绵羊DRB1基因第3外显子具有丰富的多态性;藏绵羊DRB1基因最初是由2个等位基因突变分化成两大类等位基因。
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| 关 键 词： | PCR-SSCP DRB1基因 遗传多态性 藏绵羊 |
PCR-SSCP Detection and DNA Sequence Analysis of MHC-DRB1 Gene Exon 3 in Tibetan Sheep |
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| Xu Fei,Cheng Shuru,Liu Xiu,Wang Jiqing,Hu Jiang,Luo Yuzhu.PCR-SSCP Detection and DNA Sequence Analysis of MHC-DRB1 Gene Exon 3 in Tibetan Sheep[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Xu Fei Cheng Shuru Liu Xiu Wang Jiqing Hu Jiang Luo Yuzhu |
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| Institution: | Xu Fei Cheng Shuru Liu Xiu Wang Jiqing Hu Jiang Luo Yuzhu* Gansu Key Laboratory of Herbivorous Animal Biotechnology/ Faculty of Animal Sci-Tech,GansuAgricultural University,Lanzhou 730070 China |
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| Abstract: | In order to study DRB1 gene exon 3 polymorphism in Tibetan sheep, The number of alleles, single nucleotide polymorphisms(SNPs) sites, variation type, the genetic relationship and evolutionary significance of the alleles had been analyzed. Variation in the 500 Tibetan sheep (Ovis aries) DRB1 gene exon 3 was investigated by PCR-SSCP method, followed by cloning and DNA sequencing. The results showed that 8 alleles were identified, 15 nucleotide polymorphism sites were identified in 8 sheep DRB1 gene haplotypes... |
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| Keywords: | PCR-SSCP DRB1 gene Genetic polymorphism Tibetan sheep |
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