| 牛血管生成素样蛋白4基因(ANGPTL4)编码区SNPs检测及其与南阳牛生长性状的相关性 |
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| 引用本文： | 马云,侯飞,李荣荣,马伟,蓝贤勇,陈宏.牛血管生成素样蛋白4基因(ANGPTL4)编码区SNPs检测及其与南阳牛生长性状的相关性[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 马云 侯飞 李荣荣 马伟 蓝贤勇 陈宏 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,杨凌712100;信阳师范学院生命科学学院,信阳464000
2. 西北农林科技大学动物科技学院,杨凌,712100 |
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| 基金项目： | 国家自然科学基金(No.30972080,30901023); 国家科技支撑计划(No.2008BADB2B03-19); 农业部肉牛产业技术体系专项(No.CARS-38); 国家转基因专项子课题(No.2009ZX08009-157B,No.2008ZX08007-002,No.2009ZX08007-005B-07); 河南省基础与前沿技术研究计划项目(No.092300410010)共同资助 |
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| 摘 要： | 牛血管生成素样蛋白4(Angiopoietin-like protein4,ANGPTL4)是一种与脂肪代谢相关的分泌蛋白,为研究ANGPTL4基因在调节脂肪和能量代谢中的作用,本实验采用DNA测序技术和PCR-RFLP技术,对南阳牛、郏县红牛、鲁西牛、秦川牛共计789头个体该基因的多态性进行了分析,并对检测到的多态位点与南阳牛的生长性状进行了相关性分析。多态性检测结果显示,该基因(NC_007305.4)第3和第5外显子各存在1处SNP(1422T>C,4899C>T),分别为错义和同义突变。相关性分析结果表明,2处突变位点不同基因型对南阳牛12月龄平均日增重均有显著影响(P<0.05);且第3外显子突变位点不同基因型对南阳牛12月龄体重指标也有显著影响(P<0.05)。研究结果提示,该基因2处突变位点有可能对牛的生长性状产生影响,可作为牛潜在分析育种标记。
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| 关 键 词： | 牛 血管生成素样蛋白4基因(ANGPTL4) PCR-RFLP 生长性状 SNPs |
Detection of SNPs of Bovine Angiopoietin-like protein 4 Gene(ANGPTL4) Coding Region and Its Association with Growth Traits in Nanyang Cattle |
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| Ma Yun,Hou Fei,Li Rongrong,Ma Wei,Lan Xianyong,Chen Hong.Detection of SNPs of Bovine Angiopoietin-like protein 4 Gene(ANGPTL4) Coding Region and Its Association with Growth Traits in Nanyang Cattle[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Ma Yun Hou Fei Li Rongrong Ma Wei Lan Xianyong Chen Hong |
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| Institution: | Ma Yun1,2* Hou Fei1* Li Rongrong1 Ma Wei1 Lan Xianyong1 Chen Hong1** 1 College of Animal Science and Technology,Northwest Agriculture and Forestry University,Yangling 712100,China,2 College of Life Sciences,Xinyang Normal University,Xinyang 464000,China * The authors who contribute equally |
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| Abstract: | Angiopoietin-like protein 4(ANGPTL4) is a secretory protein associated with lipid metabolism. In order to study the function of ANGPTL4 gene in the regulation fat and energy metabolism, in this study, 789 blood samples were collected from 4 bovine populations Nanyang, Jiaxian Red, Luxi and Qinchuan cattles to extract genomic DNA, and SNPs of ANGPTL4 gene were detected by DNA sequencing. Then PCR-RFLP and association analysis between detected SNPs locus and growth traits were carried out. The results indicat... |
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| Keywords: | Cattle Angiopoietin-like protein 4 gene(ANGPTL4) PCR-RFLP Growth trait SNPs |
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