| 棉花抗黄萎病基因的分子标记辅助选择研究 |
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| 引用本文： | 李志坤,张艳,王省芬,刘恒蔚,张桂寅,马峙英.棉花抗黄萎病基因的分子标记辅助选择研究[J].河北农业大学学报,2011,34(6):1-4. |
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| 作者姓名： | 李志坤 张艳 王省芬 刘恒蔚 张桂寅 马峙英 |
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| 作者单位： | 河北农业大学/河北省作物种质资源重点实验室,河北保定,071001 |
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| 基金项目： | 河北省自然科学基金(C2005000231);教育部重点科技项目(205018);河北省普通高等学校博士科研资助基金(B2004113) |
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| 摘 要： | 利用与海岛棉抗黄萎病基因连锁的SSR分子标记BNL3255-208,对海岛棉(Gossypium barbadense L.)品种‘ Pima90-53’与陆地棉(G.hirsutum L.)品种‘中棉所8号’的杂交组合后代进行了分子标记辅助选择.抗性鉴定结果表明:50个F2植株中,初步筛选到带有抗黄萎病基因分子标记的...
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| 关 键 词： | 棉花 黄萎病 分子标记辅助选择 |
Molecular marker-assisted selection for Verticilliumwilt resistance gene in cotton |
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| LI Zhi-kun
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ZHANG Yan
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WANG Xing-fen
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LIU Heng-wei
,
ZHANG Gui-yin
,
MA Zhi-ying.Molecular marker-assisted selection for Verticilliumwilt resistance gene in cotton[J].Journal of Agricultural University of Hebei,2011,34(6):1-4. |
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| Authors: | LI Zhi-kun ZHANG Yan WANG Xing-fen LIU Heng-wei ZHANG Gui-yin MA Zhi-ying |
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| Institution: | (Key Laboratory of Crop Germplasm Resources of Hebei/Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | One marker,BNL3255-208,linked with Verticillium wilt resistance gene in Gossypium barbadense cv.Pima90-53 used for marker-assisted selection in hybrid and backcrossing offspring of Pima ’90-53’(G.barbadense) and ’CCRI8’(G.hirsutum).Thirty-six of fifty plants with BNL3255-208 were detected from F2 plants.The resistance marker of 208 bp was also tested in BC1F1,with 89.4% accordance.The above results showed that the BNL3255-208 marker is effective and can be used for molecular marker-assisted breeding in cotton Verticilliam resistarce breeding. |
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| Keywords: | cotton Verticillium wilt marker-assisted selection(MAS) |
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