建立小麦品种DNA指纹的方法研究

建立小麦DNA指纹对遗传资源评价、品种权益保护、种子质量鉴定具有重要的意义。本研究采用15个SSR标记和20个AFLP-SCAR标记，分析了来自我国不同麦区的455个小麦品种，证明用两种分子标记建立小麦DNA指纹可以更加全面地反应品种的遗传多样性。从而，在提出采用SSR标记与AFLP-SCAR标记构建小麦品种DNA指纹的技术路线的基础上，建立了构建DNA指纹的方法模式。按此方法获得的品种指纹代码可以经条形码软件转换为条形码，使为每个品种建立身份证的设想成为可能。

Method of ID Constitution for Wheat Cultivars

DNA fingerprint is a useful tool for identification new cultivars,cultivars' authenticity and seed purity in wheat production and trade.In the present study,15 SSR markers and 20 AFLP-SCAR markers were employed as DNA fingerprints of 455 wheat cultivars collected from 12 provinces and eco-regions of China.The fingerprints built by the two kinds of marker could explore the cultivars' genetic diversity more reliably.Therefore,an approach to build wheat fingerprints was set up by connecting both SSR and AFLP-SCAR codes.In this paper,we exampled the way of constituting DNA fingerprints for wheat cultivars by 10 pairs of SSR marker and 10 pairs of AFLP-SCAR marker.The approach is put forward to converting DNA fingerprint code to bar code and give every wheat cultivar an ID number in the future.