| 适于SSR分析的大豆干种子中DNA快速提取 |
| |
| 引用本文： | 张伟,谢甫绨,宋显军,曹萍,郭玉华.适于SSR分析的大豆干种子中DNA快速提取[J].华北农学报,2007,22(2):133-135. |
| |
| 作者姓名： | 张伟 谢甫绨 宋显军 曹萍 郭玉华 |
| |
| 作者单位： | 沈阳农业大学,农学院,辽宁,沈阳,110161 |
| |
| 基金项目： | 引进国际先进农业科技计划(948计划) |
| |
| 摘 要： | 为了探寻大豆干种子中的DNA快速提取方法,以2个大豆品种的干种子为材料,在SDS提取液中加入蛋白酶K、NP-40、Tween-20情况下,并减少氯仿/异戊醇的抽提次数,研究对提取DNA质量的影响。结果表明,SDS提取液中加入3种试剂后,即使使用氯仿/异戊醇抽提1次,其DNA的OD260/OD280值也在1.8~2.0之间,能够满足SSR扩增模板的需求。说明此方法既可获得较高纯度的DNA,又可大大缩短大豆干种子中的DNA提取时间。
|
| 关 键 词： | 大豆 DNA提取 SDS SSR |
| 文章编号： | 1000-7091（2007）02-0133-03 |
| 修稿时间： | 2006-11-20 |
Rapid Extraction of Genome DNA from Soybean Dry Seed for SSR Analysis |
| |
| ZHANG Wei,XIE Fu-ti,SONG Xian-jan,CAO Ping,GUO Yu-hua.Rapid Extraction of Genome DNA from Soybean Dry Seed for SSR Analysis[J].Acta Agriculturae Boreali-Sinica,2007,22(2):133-135. |
| |
| Authors: | ZHANG Wei XIE Fu-ti SONG Xian-jan CAO Ping GUO Yu-hua |
| |
| Institution: | College of Agronomy, Shenyang Agricultural University, Shenyang 110161, China |
| |
| Abstract: | To develop a rapid DNA extraction method,the quality of DNA was examined with soybean dry seeds as materials,adding proteinase K,NP-40 and Tween-20 to SDS extracting solution,and reducing the chloroform/isoamyl extraction times.The results showed that when adding the three reagents to SDS extracting solution,the OD260/OD280 value of DNA was between 1.8 and 2.0 even extracted once by chloroform/isoamyl.Thus,good quality and quantity template DNA suitable for SSR analysis was obtained with reduced time. |
| |
| Keywords: | Soybean DNA extraction SDS SSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
