| 摘 要： | Objective] This study was carried out to explore the effect of poly-β-hydroxybutyrate(PHB) on the performance of the nonspecific immune system in Fenneropenaeus chinensis. Method] F. chinensis individuals were assigned into six groups, and each group was fed with a diet containing 0(Control), 0.5%(Group E_(0.5)), 1.0%(Group E_(1.0)), 2.5%(Group E_(2.5)), 5.0%(Group E_(5.0)) or 10.0% PHB(Group E_(10.0)). The mortality rate and relative percent of survival(RPS) of each group were calculated after 6 weeks. Meanwhile, the total antioxidant capacity(T-AOC), the activity of acid phosphatase(ACP), peroxidase(POD) and catalase(CAT), and the content of malondialdehyde(MDA) in hepatopancreas and serum were measured,and their correlation with PHB concentration was analyzed. Result] The RPS in PHB treated shrimps increased initially and decreased subsequently with increasing PHB concentration. RPS of Group E_(1.0) was the highest, showing significant difference from that of other groups( P 0.05).With the increase in PHB concentration, the activity of immunity related enzymes changed in a similar pattern with RPS: increasing at first and decreasing subsequently. In addition, the activity of the enzymes was elevated in the 2~(nd) and 3~(rd) weeks of PHB administration. Among them, T-AOC in serum of groups E_(1.0) and E_(2.5), T-AOC in hepatopancreas of Group E_(1.0), ACP activity in serum of groups E_(1.0) and E_(2.5), ACP activity in hepatopancreas of Group E_(1.0), CAT activity in groups E_(0.5), E_(1.0) and E_(2.5), CAT activity in hepatopancreas of groups E_(0.5), E_(1.0) and E_(10.0), POD activity in both serum and hepatopancreas of groups E_(0.5), E_(1.0) and E_(2.5), SOD activity in both serum and hepatopancreas of Group E_(1.0), MDA content in serum of Group E_(1.0) and MDA content in hepatopancreas of groups E_(0.5) and E_(1.0) showed significant difference from those of other groups(P0.05). Conclusion] PHB can improve the immunity of F. chinensis, 1.0% in feed has the best effect, and the total enzyme activity reaches the highest level in the 2~(nd) and 3~(rd) weeks of PHB administration.
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