贮藏温度和时间对木棉与美丽异木棉种子活力的影响

利用木棉 Bombax ceiba 和美丽异木棉Chorisia speciosa混合种子,在0、4、和8℃条件下贮藏135d,每隔45 d测定一次种子场圃发芽率和活力指标,用2,3,5-三苯基氯化四氮唑法(TTC法)测定种子的初始和贮藏135 d的生活力。结果如下:木棉种子在相同贮藏温度条件下,随贮藏时间的延长,发芽率、发芽势、活力指数存在显著差异(P<0.05),贮藏温度和贮藏时间有极显著的交互作用,种子活力逐渐下降,贮藏135 d后种子发芽率在8.33%以下,基本失去发芽能力;美丽异木棉种子在不同温度下,随着贮藏时间延长,发芽率、发芽势、活力指数没有显著差异,贮藏温度和贮藏时间没有显著的交互作用,种子发芽率为新鲜种子发芽率的67.46%以上;利用TTC染色法(1.0%浓度)能反映出木棉的种子生活力;木棉种子0℃贮藏90 d发芽率好于4和8℃,可在生产中推广应用TTC染色法检测木棉的种子生活力;美丽异木棉种子0、4、8℃贮藏135 d的发芽率差异很小,0℃贮藏发芽率和活力指数较优。

Effects of Storage Temperature and Time on Seed Vigor of Bombax ceiba and Chorisia speciosa

Through the analysis of different storage temperature and time on the germination and vigor index of the Bombax ceiba and Chorisia speciosa seeds,we aim to explore the effect of seed storage temperature and time on the germination rate and seed vigor of the two species.Using the mixed seeds of B.ceiba and C.speciosa,stored at 0,4 and 8℃for 135 days,we tested the germination rate and vigor index of the seeds in every 45 days.TTC was used to test the seed vigor at the initial time and after 135 days storage.Under the same storage temperature,the germination rate,germination potential and vitality index of B.ceiba seeds were significantly different with the extension of storage time.The storage temperature and storage time had very significant interaction,the seed vigor gradually decreased with the storage prolonged and the temperature increased.After 135 days storage,the germination rate of B.ceiba seeds is below 8.33%,and the germination ability is basically lost.On the other hand,there is no significant difference in germination rate,germination vigor and vigor index of the seeds of C.speciosa after 135 days storage.There is no significant interaction between storage temperature and storage time in the C.speciosa seeds germination.After 135 days of storage,the germination rate decreased to 67.47%of initial.Using the 1.0%TTC dyeing method can accurately reflect the seed vigor of B.ceiba.The germination rate of B.ceiba seeds stored at 0 for 90 days is better than 4 and 8℃.We can popularize the application of TTC dyeing method to detect the seed vigor of B.ceiba.The best temperature to store the C.speciosa is 0°C and the difference in germination rate of the seeds stored at 0、4、8℃is small.