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聚乙烯醇在草莓苗组织培养中的作用
作者姓名:刘雪静  代 丽  尹宝重  
作者单位:刘雪静,尹宝重,Liu Xue-jing,Yin Bao-zhong(河北农业大学植物保护学院,河北保定,071001);代丽,Dai Li(河北农业大学园艺学院,河北保定,071001);甄文超,Zhen Wen-chao(河北农业大学植物保护学院,河北保定,071001;河北省农作物病虫害生物防治工程技术中心,河北保定,071001) ?
基金项目:教育部科学技术研究重点项目,河北省自然科学基金重点项目?
摘    要:以丰香草莓组培苗为试材,研究了不同浓度聚乙烯醇对草莓组培苗叶片愈伤组织诱导、继代增殖及生根培养的影响.结果表明,1 g/L的聚乙烯醇可使草莓组培苗叶片愈伤组织发生期提前,提高愈伤组织发生率和发生量,改变愈伤组织的质量,同时能有效降低草莓组培苗在继代增殖中的玻璃化现象,促进植株分化、提高叶绿素和蛋白的含量.2 g/L的聚乙烯醇可以提高组培苗在生根培养基中的生根条数、根长度、株高、叶绿素和蛋白含量等.

关 键 词:草莓  聚乙烯醇  组织培养

Effects of Polyvinyl alcohol on tissue culture of Strawberry
Authors:Liu Xue-jing  Dai Li  Yin Bao-zhong  Zhen Wen-chao
Abstract:The tissue culture of Fengxiang strawberry was used as experimental materials. The effects of PVA on tissue culture of strawberry were studied. The results indicated that 1g/L PVA can reduce the rate of vitrified plantlets of strawberry significantly during proliferating, and can also improve the contenes of chlorophyll and albumen. During leaf culture, 1g/L PVA may quicken callus emergence and increase the rate of callus formation and improve the quality of callus. 2g/L PVA can improve the root number of single plantlet, the length of roots, the high of plantlet, the contents of chlorophyll and albumen.
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