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| 鸡外周血淋巴细胞培养及转移因子的提取 | |
| 引用本文： | 毕井超,宋晓林,齐旭阳,李凤华,涂岳,庄国宏,江国托,余丽芸.鸡外周血淋巴细胞培养及转移因子的提取[J].黑龙江八一农垦大学学报,2006,18(4):54-57. |
| 作者姓名： | 毕井超 宋晓林 齐旭阳 李凤华 涂岳 庄国宏 江国托 余丽芸 |
| 作者单位： | 1. 黑龙江八一农垦大学生命科技学院,大庆,163319 2. 大连三仪生物工程研究所 3. 大连轻工业学院生物与食品工程学院 |
| 基金项目： | 大连三仪动物药品有限公司资助项目 |
| 摘 要： | 转移因子(TransferFactor,简称TF)是白细胞中具有免疫活性的T淋巴细胞所释放的一类小分子可透析物质。在传统转移因子制备方法的基础上,采用鸡外周血淋巴细胞培养法研制转移因子,并对制备转移因子中的核糖含量进行测定。结果表明:连续传代4次的淋巴细胞可以提取转移因子,转移因子的浓度较高。 |
| 关 键 词： | 外周血 细胞培养 转移因子 含量测定 |
| 文章编号： | 1002-2090（2006）04-0054-04 |
| 收稿时间： | 2006-04-23 |
| 修稿时间： | 2006-04-23 |
Cultivation of Chicken Peripheral Blood Lymphocytes Cells and Extraction of The Transfer Factors |
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| BI Jing-chao, SONG Xiao-lin, QI Xu-yang et al..Cultivation of Chicken Peripheral Blood Lymphocytes Cells and Extraction of The Transfer Factors[J].Journal of Heilongjiang August First Land Reclamation University,2006,18(4):54-57. | |
| Authors: | BI Jing-chao SONG Xiao-lin QI Xu-yang |
| Abstract: | The transfer factor released form T lymphocytes in chicken leukocyte, which had immunity activity, was a kind of small molecular dialyzable substance. The cultivation of chicken peripheral blood lymphocytes cells was performed to prepare the transfer factors basing on the traditional method. The content in these transfer factors was measured. The results showed that the TF could be extracted from the four generations cultured lymphocytes and the content of these extracted TF was higher. |
| Keywords: | peripheral blood cell culture transfer factor content measurement |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |