农杆菌侵染玉米萌动胚转化抗逆基因BcBCP1

以5份优良玉米自交系为试材,采用农杆菌侵染玉米萌动胚的方法转化蓝铜蛋白类似基因BcBCP1,同时优化遗传转化体系。结果表明,菌液浓度为OD600=0.8、侵染24h、共培养3d时,遗传转化率分别达到最高;不同基因型的遗传转化率存在差异,其中丹598转化率最高,达到6.67%;丹599的遗传转化率最低,为0.56%,5个不同基因型的平均遗传转化率为2.13%。共获得PCR阳性植株43株,其中28株结实。

Introduction of BcBCP1 Gene Mediated by Agrobacterium Tumefacien into Germinating Embryo of Maize

BcBCP1 gene was introduced into 5 maize inbred lines by Agrobacterium Tumefacien pathway using germinating embryo as receptor and the influence factors of genetic transformation was optimize at the same time. The results indicated that the rate of transformation reached the maximum when the concentration of bacterium was OD₆₀₀ was 0.8, the infection time was 24h and the co-cultivation time was three days. There were different transformation rates among maize genotypes and the average of it was 2.13%. Forty-three transformed plants were positive by PCR, and 28 plants among them were seed.