干酪乳杆菌干酪亚种Lactobacillus casei subsp.casei SY13对衰老模型小鼠的抗氧化作用

 【目的】利用一株经体外筛选具有较强抗氧化活性的干酪乳杆菌干酪亚种(Lactobacillus casei subsp.casei)SY13活菌制剂和灭活制剂研究D-半乳糖所致衰老模型小鼠体内抗氧化能力。【方法】 采用SY13活菌制剂和灭活制剂饲喂由D-半乳糖连续皮下注射诱导的亚急性衰老模型小鼠,以各组小鼠血清和肝组织匀浆中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力、总抗氧化能力(T-AOC)及脂质过氧化产物丙二醛(MDA)的含量为指标,全面评价其在小鼠体内的抗氧化能力。【结果】 Lactobacillus casei subsp. casei SY13活菌制剂高、中剂量组(20、10 mL•kg-1)能降低连续6周注射D-半乳糖诱发的衰老小鼠血清和肝脏组织中的MDA含量,同时提高血清和肝脏组织中GSH-Px活力以及总抗氧化能力(T-AOC),SOD活力变化不明显。【结论】 SY13活菌制剂能有效提高亚急性衰老小鼠的抗氧化能力,具有一定延缓衰老的作用。

Antioxidative Activity of Lactobacillus casei subsp. casei SY_(13) on Ageing Model Mice

Objective] The objective of the experiment was to study the antioxidative activity of viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13 on aged model mice induced by D-galactose.Method] ICR subacute aged model mice,which were given subcutaneous injection of D-galactose continuously,were fed with viable cells and heat-killing cells of Lactobacillus casei subsp.casei SY13.The content of malondialdehyde(MDA),activity of superoxide dismutasc(SOD),glutathione peroxidase(GSH-Px)and total antioxidant capacity(T-AOC)in serum and liver of the mice were tested.Result] SY13 viable cells high dose group and middle dose group(20,10 mL·kg-1)could obviously reduce the aged mice serum and liver tissue MDA content,enhance evidently the GSH-Px activity and total antioxidant capacity(T-AOC),but the SOD activity did not change significantly.Conclusion] Lactobacillus casei subsp.casei SY13 viable cells Can obviously increase the antioxidant ability of ageing mice and has certain anti-aging effects.