两种农药树干注射对垂柳叶内PPO和POD活性及同工酶谱的影响

研究了吡虫啉和啶虫脒树干注射对垂柳叶内多酚氧化酶(PPO)、过氧化物酶(POD)活性及其同工酶谱变化的影响。结果表明,两种杀虫剂树干注射均可引起垂柳叶片PPO活性上升,POD活性下降。吡虫啉处理对PPO和POD活性的影响均大于啶虫脒,两种药剂处理后15 d PPO活性均达到最大值,10 d POD活性均最低,药后30 d PPO和POD活性逐渐恢复到对照水平。吡虫啉处理使PPO同工酶酶带E1(Rf=0．71)消失,E2(Rf=0．77)活性升高;啶虫脒处理使PPO同工酶酶带E1活性升高,E2消失;药后10,45 d各处理酶带带型基本稳定。吡虫啉处理使POD同工酶药后10 d酶带P1(Rf=0．51)、P4(Rf=0．67)消失,45 d P1消失而P4又出现;啶虫脒处理使POD同工酶酶带P1活性升高,P2(Rf=0．56)消失,药后10,45 d酶带带型相同。说明PPO和POD活性变化及其同工酶谱变化与注药种类有密切关系,可作为研究树干注药对树体生理影响的指标。

Effects of two pesticides on PPO, POD activity and isozymes in weeping willow (Salix babylonica Linn) by trunk injection

The paper reported the effects of imidacolprid and acetamiprid on polyphenol (PPO), perox-idase (POD) activity and isozymes in weeping willow (Salix babylonica L. ) leaves through trunk injection. Results showed that the two pesticides affected activities of PPO and POD as well as spectrums and activities of their isozymes after trunk injection; the activity of PPO was increased, while the activity of POD was decreased. Imidacolprid had more effect on the activity of PPO, POD than acetamiprid. After treatment of 15 days,the activity of PPO reached max,while after treatment of 10 days,the activity of POD reached minimam. The activities of PPO and POD resumed the normal level after 30 days of treatment. Imidacolprid caused the disappearance of El ( Rf = 0. 71 ) and the increased activity of E2 ( Rf = 0. 77 ) of PPO isozymes. Acetamiprid caused the increased activity of El and the disappearance of E2 of PPO isozymes. The changes of spectrums of PPO isozymes after 10 days were as stable as those after 45 days af- ter trunk injection with two pesticides. Imidacolprid caused the disappearance of PI ( Rf = 0. 51 ) and P4 . ( Rf = 0. 67 ) after 10 days but appearance of P4 after 45 days of POD isozymes. Acetamiprid caused the increased activity of P1 ,and the disappearance of P2 ( Rf = 0. 56 ) of POD isozymes. The effects of spectrums of POD isozymes after 10 days were the same as those after 45 days by trunk injection with acetamiprid. It was postulated that the activities of PPO,POD and their isozymes could be used as indicators to research the effects on physiology of trees through trunk infection.