| Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum |
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| 作者姓名： | WANG Han-yan WEI Yu-ming ZE Hong-yan ZHENG You-liang |
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| 作者单位： | [1]Triticeae Research Institute, Sichuan Agricultural University, Ya＇an 625014, P.R.China [2]Key Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education/Sichuan Agricultural University,Ya＇an 625014, P,R. China |
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| 基金项目： | This work was supported by the National High Technology Research and Development Program of China (863 Program, 2006AA10Z179, 2006AA10ZEF87), National Natural Science Foundation of China (30300219 and 30571163),and the Foundation for the Authors of
National Excellent Doctoral Dissertation of China
(200357 and 200458) |
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| 摘 要： | Three coding sequences of gliadins genes, designed as Gli2_Dul, Gli2_Du2 and Gli2_Du3, were isolated from the genomic DNA of Triticum durum accessions CItr5083. Gli2_Dul and Gli2_Du2 contain 945 and 864 bp, encoding the mature proteins with 314 and 287 amino acid residues, respectively. Gli2_Du3 is recognized as a pseudogene due to the stop codon occurring in the coding region. The pseudogenes, commonly occurring in gliadins family, are attributed to the single base change C→T. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-gliadin proteins, including the toxic sequences （PSQQQP）. The peptide fraction PF（Y）PP（Q）is thought to be an extra unit of repetitive domain, slightly diverging from the previous report. Six cysteine residues were observed within two unique domains. Phylogenetic analysis showed Gli2_Du2 and Gli2_Du3 were closely related to the genes on chromosome 6A, whereas Gli2_Dul seems to be more homologous with the genes on chromosome 6B.
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| 关 键 词： | 硬粒小麦 α-醇溶蛋白 基因克隆 序列分析 |
| 收稿时间： | 2006-06-29 |
| 修稿时间： | 2006-08-16 |
Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum |
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| WANG Han-yan WEI Yu-ming ZE Hong-yan ZHENG You-liang.Isolation and Analysis of α-Gliadin Gene Coding Sequences from Triticum durum[J].Agricultural Sciences in China,2007,6(1):25-32. |
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| Authors: | Han-yan WANG Yu-ming WEI Hong-yan ZE You-liang ZHENG |
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| Institution: | aTriticeae Research Institute, Sichuan Agricultural University, Ya'an 625014, P.R. China;bKey Laboratory of Crop Genetic Resources and Improvement in Southwest China, Ministry of Education/Sichuan Agricultural University, Ya'an 625014, P.R. China |
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| Abstract: | Three coding sequences of gliadins genes, designed as Gli2_Du1, Gli2_Du2 and Gli2_Du3, were isolated from the genomic DNA of Triticum durum accessions CItr5083. Gli2_Du1 and Gli2_Du2 contain 945 and 864 bp, encoding the mature proteins with 314 and 287 amino acid residues, respectively. Gli2_Du3 is recognized as a pseudogene due to the stop codon occurring in the coding region. The pseudogenes, commonly occurring in gliadins family, are attributed to the single base change C → T. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-gliadin proteins, including the toxic sequences (PSQQQP). The peptide fraction PF(Y)PP(Q) is thought to be an extra unit of repetitive domain, slightly diverging from the previous report. Six cysteine residues were observed within two unique domains. Phylogenetic analysis showed Gli2_Du2 and Gli2_Du3 were closely related to the genes on chromosome 6A, whereas Gli2_Du1 seems to be more homologous with the genes on chromosome 6B. |
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| Keywords: | durum wheat α -gliadin gene clone sequence analysis |
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