| 与黄瓜M基因连锁的三个共显性标记 |
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| 引用本文： | 时秋香,刘世强,李征,曹辰兴,李颖,黄三文.与黄瓜M基因连锁的三个共显性标记[J].园艺学报,2009,36(5):737-742. |
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| 作者姓名： | 时秋香 刘世强 李征 曹辰兴 李颖 黄三文 |
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| 作者单位： | （山东农业大学园艺科学与工程学院，山东泰安271000；中国农业科学院蔬菜花卉研究所，北京100081；上海交通大学农业与生物学院，上海200240） |
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| 基金项目： | 国家自然科学基金，农业部引进国际先进农业科学技术计划(948计划) |
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| 摘 要： | 利用黄瓜近等基因系纯雌株WI1983G（基因型为FFMM）与两性花株WI1983H（基因型为FFMM）为亲本，以WI1983H为回交亲本构建了BC1代分离群体，采用SSR及SCAR分子标记技术，获得了3个与M基因紧密连锁的标记SSR23487、SCAR123和SSR19914，它们与M基因的遗传距离分别为0.28 cM、0.94 cM和3.20 cM，两侧标记SSR23487和SCAR123将M基因定位在1.22 cM的遗传区间内。这3个分子标记的获得不仅可以用于分子标记辅助选择育种，而且为最终M基因的克隆打下了基础。
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| 关 键 词： | 黄瓜 分子标记 性别表达 SSR |
| 收稿时间： | 2008-12-22 |
Three Co-dominant Markers Linked to M Gene in Cucumis sativus |
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| SHI Qiu-xiang,LIU Shi-qiang,LI Zheng,CAO Chen-xing,LI Ying,HUANG San-wen.Three Co-dominant Markers Linked to M Gene in Cucumis sativus[J].Acta Horticulturae Sinica,2009,36(5):737-742. |
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| Authors: | SHI Qiu-xiang LIU Shi-qiang LI Zheng CAO Chen-xing LI Ying HUANG San-wen |
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| Institution: | (College of Horticulture Science and Engineering，Shandong Agricultural University，Tai'an 271000，China；Institute of Vegetable and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；School of Agriculture and biology，Shanghai Jiaotong University，Shanghai 200240，China) |
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| Abstract: | A segregating cucumber BC1 population was developed using the nearly isogenic lines WI1983G (gynoecious, FFMM) as donor and WI1983H (hermaphroditic, FFMM) as recurrent parent. With screening of polymorphism of one SCAR and, 2112 SSR markers, three polymorphic co-dominant markers SSR23487, SCAR123 and SSR19914 were found to be tightly linking to the M/m gene，with a distance of 0.28 cM, 0.94 cM and 3.20 cM respectively. The interval between SSR23487 and SCAR123, which locate on each side of the M/m gene, was 1.22 cM. These markers not only be useful in markers-assisted selection for gynoecious plant, but also provide a solid basis for isolation of the sex-determining M/m gene in cucumber. |
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| Keywords: | SSR |
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