| 紫外-光复活诱变香兰素生产菌株的研究 |
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| 引用本文： | 周庆礼,王洪亮.紫外-光复活诱变香兰素生产菌株的研究[J].安徽农业科学,2008,36(4):1292-1294. |
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| 作者姓名： | 周庆礼 王洪亮 |
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| 作者单位： | 1. 陕西科技大学生命科学与工程学院,陕西西安,710021;天津科技大学,天津,300457
2. 陕西科技大学生命科学与工程学院,陕西西安,710021 |
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| 基金项目： | 2005国家自然科学基金（20576066）;2003年度天津市科技攻关项目重点应用基础研究项目（03382211）. |
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| 摘 要： | 目的]探究紫外诱变和光复活修复对生产香兰素菌株的效果。方法]通过紫外诱变和光复活修复对香兰素生产菌链霉菌(Streptomycessp.L1936)进行复合诱变,筛选出香兰素高产菌株(Streptomycessp.L1936-9)。结果]出发菌株经过紫外诱变和光复活修复处理后,脱乙酰酶酶活提高了71.1%,香兰素氧化酶酶活降低了34.6%相应的香兰素产量提高了34.2%。结论]该研究为香兰素生产提供了良好途径。
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| 关 键 词： | 香兰素 链霉菌 紫外-光复活诱变 |
| 文章编号： | 0517-6611（2008）04-01292-03 |
| 收稿时间： | 2007-09-26 |
| 修稿时间： | 2007年9月26日 |
Study on Inducing the Strain Producing Vanillin by UV-photoreactivation |
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| ZHOU Qing-li et al.Study on Inducing the Strain Producing Vanillin by UV-photoreactivation[J].Journal of Anhui Agricultural Sciences,2008,36(4):1292-1294. |
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| Authors: | ZHOU Qing-li |
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| Abstract: | Objective] The purpose was to research into the effects of UV mutagenesis and photoreactivation repair on the strain producing vanillin.Method] Streptomyces sp.L 1936 producing vanillin was conducted for compound mutagenesis by UV mutagenesis and photoreactivation so as to screen out the high-yield strain of vanillin(Streptomyces sp.L1936-9).Result] After the initial strain was treated with UV mutagenesis and photoreactivation repair,the deacetylase activity was enhanced by 71.1%,the vanillin oxidase activity was decreased by 34.6% and the corresponding vanillin yield was enhanced by 34.2% in the strain.Conclusion] The research provided good approach for vanillin production. |
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| Keywords: | Vanillin Streptomyces UV-photoreactivation mutagenesis |
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