| 5个重庆地方猪种遗传多样性的微卫星分析 |
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| 引用本文： | 白小青,范首君,王金勇,刘文,郭宗义,何道领,陈四清,王可甜,付文贵,陈磊,赵久刚,蓝静,张亮. 5个重庆地方猪种遗传多样性的微卫星分析[J]. 畜牧兽医学报, 2010, 41(12) |
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| 作者姓名： | 白小青 范首君 王金勇 刘文 郭宗义 何道领 陈四清 王可甜 付文贵 陈磊 赵久刚 蓝静 张亮 |
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| 作者单位： | 重庆市畜牧科学院;重庆市畜牧技术推广总站; |
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| 基金项目： | 重庆市自然科学重点基金项目，重庆市重大科技攻关项目，国家十一五科技支撑计划项目 |
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| 摘 要： | 为了分析5个重庆地方猪种的遗传多样性和系统发生关系,采用FAO-ISAG联合推荐的27个微卫星标记,以有效等位基因数、期望杂合度和多态信息含量等遗传参数为指标,评估了品种内遗传变异。计算了F统计量、Nei氏遗传距离(DA)和Nei氏标准遗传距离(DS)并进行UPGMA和NJ聚类分析及主成分分析,进而探讨了品种间的遗传分化和亲缘关系。结果,27个微卫星座位共检测到542个等位基因,其中43个等位基因为单一品种所特有。5个猪种的遗传多样性丰富:有效等位基因数(Ne)在9.4933～11.0280之间,期望杂合度(He)在0.8897～0.9091之间,多态信息含量(PIC)在0.8689～0.8926之间。F统计量分析表明,重庆地方猪种遗传分化明显(P0.001),各群体内存在一定程度的近交。2种聚类方法以NJ法更为可靠。5个猪种聚为两类,荣昌猪、渠溪猪和合川猪为一类,罗盘山猪和盆周山地猪为一类。相关性分析表明,遗传距离与地理距离间无显著的相关(P0.05)。该研究结果为重庆地方猪种的保护和利用提供了重要的理论依据。
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| 关 键 词： | 地方猪种 遗传多样性 微卫星标记 遗传距离 聚类分析 |
Genetic Diversity of Five Native Pig Breeds of Chongqing Based on Microsatellite DNA Markers |
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| BAI Xiao-qing,FAN Shou-jun,WANG Jin-yong,LIU Wen,GUO Zong-yi,HE Dao-ling,CHEN Si-qing,WANG Ke-tian,FU Wen-gui,CHEN Lei,ZHAO Jiu-gang,LAN Jing,ZHANG Liang. Genetic Diversity of Five Native Pig Breeds of Chongqing Based on Microsatellite DNA Markers[J]. Chinese Journal of Animal and Veterinary Sciences, 2010, 41(12) |
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| Authors: | BAI Xiao-qing FAN Shou-jun WANG Jin-yong LIU Wen GUO Zong-yi HE Dao-ling CHEN Si-qing WANG Ke-tian FU Wen-gui CHEN Lei ZHAO Jiu-gang LAN Jing ZHANG Liang |
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| Affiliation: | BAI Xiao-qing1,FAN Shou-jun2,WANG Jin-yong1*,LIU Wen1,GUO Zong-yi1,HE Dao-ling2,CHEN Si-qing1,WANG Ke-tian1,FU Wen-gui1,CHEN Lei1,ZHAO Jiu-gang1,LAN Jing1,ZHANG Liang1(1.Chongqing Academy of Animal Sciences,Rongchang 402460,China,2.Chongqing General Demonstration Station of Animal Husbandry Technology,Yubei 401121,China) |
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| Abstract: | The study was conducted to analyze the genetic diversity and phylogenetics of five Chongqing indigenous pig breeds in Chongqing.Using 27 microsatellite markers recommended by the International Society for Animal Genetics(ISAG)and Food and Agriculture Organization(FAO),by effective number of alleles,expected heterozygosity,polymorphism information content and other indexs,the genetic variability within pig breeds were estimated.By F-statistic,Nei's genetic distance,Nei's standard genetic distance,dendrogram ... |
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| Keywords: | indigenous pig breeds genetic diversity microsatellite marker genetic distance dendrogram analysis |
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