| 葡萄属植物染色体DNA的提取、纯化及RFLP鉴定 |
| |
| 引用本文： | 张立平,林伯年,沈德绪,董继新.葡萄属植物染色体DNA的提取、纯化及RFLP鉴定[J].果树学报,1996(2). |
| |
| 作者姓名： | 张立平 林伯年 沈德绪 董继新 |
| |
| 作者单位： | 浙江农业大学园艺系 |
| |
| 摘 要： | 利用ＪｅｎｎｉｆｅｒＡ．ＣｏｕｃｈａｎｄＰａｕｌＪ．Ｆｒｉｔｚ的改良方法提取葡萄嫩叶染色体ＤＮＡ，并进行ＲＦＬＰ分析。结果表明，该方法提取的ＤＮＡ均未降解；以Ｓｅｐｈａｒｏｓｅ２Ｂ层析柱纯化后可进行ＲＦＬＰ分析；核糖体ｒＤＮＡ的ＰＶｖｃ一Ａ片段制备的探针与ＨｉｎｄⅢ酶组合，使所有供试样品都显示多态性。
|
| 关 键 词： | 葡萄属 DNA RFLP |
Isolation,Purification of Genomic DNA and Identification of RFLP in Vitis |
| |
| Zhang Liping, Lin
Bonian, Shen Dexu, and Dong Jixin.Isolation,Purification of Genomic DNA and Identification of RFLP in Vitis[J].Journal of Fruit Science,1996(2). |
| |
| Authors: | Zhang Liping Lin
Bonian Shen Dexu and Dong Jixin |
| |
| Abstract: | Genomic DNA had been isolated from young
grapevine leaves according to Jennifer and Paul's modifiedmethod and purified by sepharose 2B
chromatography,and then successfully used in RFLP analysis.Resultshowed that the combination
of PVvc一A/HindⅢ was an effective method used in identifing all tested speciesand cultivars by
polymorphism. |
| |
| Keywords: | itis DNA RFLP |
| 本文献已被 CNKI 等数据库收录！ |
|
