外源H2O2对复盐胁迫下木槿的生理响应机制

以当年生木槿幼苗为试材,采用盆栽法,研究了外源H₂O₂对木槿在复盐胁迫下的生理指标的影响,以期为其寻找新的耐盐途径、提高其耐盐性提供参考依据。结果表明:T4与T2相比显著降低了木槿超氧阴离子、过氧化氢、丙二醛(MDA)含量和电导率,T2与对照相比差异显著,T6与对照之间无显著差异;T4与T2相比提高了抗坏血酸(AsA)含量和谷胱甘肽(GSH)含量,T2与T1之间差异显著;T4与T2相比显著提高了木槿超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性,T2显著高于对照,T6与对照之间无显著差异;T4与T2相比提高了木槿可溶性糖、可溶性蛋白质、游离脯氨酸的含量,T2显著高于对照;T4与T2相比提高了叶绿素a含量和叶绿素a/b值,降低了叶绿素b和总叶绿素含量。综合分析认为,T4对改善复盐胁迫下木槿生理特性和缓解复盐胁迫效果最显著。

Physiological Response Mechanism of Exogenous H2O2 to Hibiscus syriacus Linn.Under Double Salt Stress

The seedlings of Hibiscus syriacus Linn.were used as materials,the effects of exogenous H₂O₂ on physiological indexes of Hibiscus syriacus Linn.under double salt stress were studied by pot experiment,in order to find a new way to improve its salt tolerance and to provide a theoretical basis.The results showed that T4 compared with T2 was significantly reduced the contents of Hibiscus syriacus Linn.superoxide anion,hydrogen peroxide and MDA content,and it was significantly reduced the conductivity,there was significant difference between T2 and control,but no significant difference between T6 and control;T4 compared with T2 that increased the content of AsA and GSH,there was significant difference between T2 and T1;T4 compared with T2 that significantly increased the activity of SOD,CAT and APX in Hibiscus syriacus Linn.,T2 was significantly higher than the control,and there was no significant difference between T6 and the control;T4 compared with T2 that increased the content of soluble sugar,soluble protein and free proline content in Hibiscus syriacus Linn.,T2 was significantly higher than the control;T4 compared with T2 that increases chlorophyll a content and chlorophyll a/b value,while decreases chlorophyll b and total chlorophyll content.According to the comprehensive analysis,T4 had the most significant effect on improving the physiological characteristics of Hibiscus syriacus Linn.under double salt stress and alleviating double salt stress.